LncRNA PART1 Promotes Breast Cancer Cell Progression by Directly Targeting MiR-4516

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Sep 14

PMID 32922076

Citations 14

Authors

Zhuo Wang

Ruqing Xu

Affiliations

Soon will be listed here.

Abstract

Introduction: Breast cancer is a serious threat to human health. It is meaningful to study the pathogenesis of breast cancer. lncRNAs have been found to play vital roles in numerous biological processes including development, immunology and cancer.

Methods: qRT-PCR was performed to examine the expressions of PART1 and miR-4516. CCK-8 assay, colony formation assay and transwell assay were used to examine the progression of breast cancer cells.

Results: In this study, we showed that lncRNA PART1 was highly expressed in breast cancer cells. Knockdown of PART1 induced decreased proliferation, invasion and migration of breast cancer cells. Moreover, we found that PART1 can bind to miR-4516 directly. We also found that inhibition of miR-4516 could rescue the decreased proliferation, migration and invasion of breast cancer cells induced by knockdown of PART1.

Discussion: lncRNA PART1 and miR-4516 were proven to be involved in the progression of many cancers. However, the roles of lncRNA PART1 and miR-4516 in the regulation of breast cancer remain unknown. Here, we demonstrated that PART1 can bind to miR-4516 to decrease the expression of miR-4516 and promote the development of breast cancer.

Citing Articles

Exosomal miR-6126 as a novel therapeutic target for overcoming resistance of anti-cancer effect in hepatocellular carcinoma.

Hwang H, Kim J, Kim T, Han Y, Choi D, Cho S BMC Cancer. 2024; 24(1):1557.

PMID: 39702014 PMC: 11660897. DOI: 10.1186/s12885-024-13342-y.

PART1 facilitates tumorigenesis and inhibits ferroptosis by regulating the miR-490-3p/SLC7A11 axis in hepatocellular carcinoma.

Li D, Wan M, Liu X, Ojha S, Sheng Y, Li Y Aging (Albany NY). 2024; 16(14):11339-11358.

PMID: 39029955 PMC: 11315397. DOI: 10.18632/aging.206009.

Temporin-GHaK Exhibits Antineoplastic Activity against Human Lung Adenocarcinoma by Inhibiting the Wnt Signaling Pathway through miRNA-4516.

Liu Y, Liu H, Zhang J, Zhang Y Molecules. 2024; 29(12).

PMID: 38930863 PMC: 11206823. DOI: 10.3390/molecules29122797.

DAGLβ is the principal synthesizing enzyme of 2-AG and promotes aggressive phenotype of intrahepatic cholangiocarcinoma via AP-1/DAGLβ/miR4516 feedforward circuitry.

Ma M, Zeng G, Tan B, Zhao G, Su Q, Zhang W Am J Physiol Gastrointest Liver Physiol. 2023; 325(3):G213-G229.

PMID: 37366545 PMC: 10435072. DOI: 10.1152/ajpgi.00243.2022.

A review on the role of long non-coding RNA prostate androgen-regulated transcript 1 (PART1) in the etiology of different disorders.

Ghafouri-Fard S, Harsij A, Hussen B, Abdullah S, Baniahmad A, Taheri M Front Cell Dev Biol. 2023; 11:1124615.

PMID: 36875771 PMC: 9974648. DOI: 10.3389/fcell.2023.1124615.

References

Kong Q, Qiu M . Long noncoding RNA SNHG15 promotes human breast cancer proliferation, migration and invasion by sponging miR-211-3p. Biochem Biophys Res Commun. 2017; 495(2):1594-1600. DOI: 10.1016/j.bbrc.2017.12.013. View

Chowdhari S, Saini N . hsa-miR-4516 mediated downregulation of STAT3/CDK6/UBE2N plays a role in PUVA induced apoptosis in keratinocytes. J Cell Physiol. 2014; 229(11):1630-8. DOI: 10.1002/jcp.24608. View

Li M, Zhang W, Zhang S, Wang C, Lin Y . PART1 expression is associated with poor prognosis and tumor recurrence in stage I-III non-small cell lung cancer. J Cancer. 2017; 8(10):1795-1800. PMC: 5556642. DOI: 10.7150/jca.18848. View

Kang M, Ren M, Li Y, Fu Y, Deng M, Li C . Exosome-mediated transfer of lncRNA PART1 induces gefitinib resistance in esophageal squamous cell carcinoma via functioning as a competing endogenous RNA. J Exp Clin Cancer Res. 2018; 37(1):171. PMC: 6063009. DOI: 10.1186/s13046-018-0845-9. View

Wang J, Liu H, Li M . Downregulation of miR-505 promotes cell proliferation, migration and invasion, and predicts poor prognosis in breast cancer. Oncol Lett. 2019; 18(1):247-254. PMC: 6540292. DOI: 10.3892/ol.2019.10334. View

Rion N, Ruegg M . LncRNA-encoded peptides: More than translational noise?. Cell Res. 2017; 27(5):604-605. PMC: 5520851. DOI: 10.1038/cr.2017.35. View

Liu M, Gou L, Xia J, Wan Q, Jiang Y, Sun S . LncRNA ITGB2-AS1 Could Promote the Migration and Invasion of Breast Cancer Cells through Up-Regulating ITGB2. Int J Mol Sci. 2018; 19(7). PMC: 6073814. DOI: 10.3390/ijms19071866. View

Jiang R, Zhao C, Gao B, Xu J, Song W, Shi P . Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer. Int J Biochem Cell Biol. 2018; 102:1-9. DOI: 10.1016/j.biocel.2018.06.003. View

Cai C, Huo Q, Wang X, Chen B, Yang Q . SNHG16 contributes to breast cancer cell migration by competitively binding miR-98 with E2F5. Biochem Biophys Res Commun. 2017; 485(2):272-278. DOI: 10.1016/j.bbrc.2017.02.094. View

10.

Wang Y, Zhou J, Wang Z, Wang P, Li S . Upregulation of SOX2 activated LncRNA PVT1 expression promotes breast cancer cell growth and invasion. Biochem Biophys Res Commun. 2017; 493(1):429-436. DOI: 10.1016/j.bbrc.2017.09.005. View

11.

Nazir S, Kumar R, Singh A, Khan A, Tanwar P, Tripathi R . Breast cancer invasion and progression by MMP-9 through Ets-1 transcription factor. Gene. 2019; 711:143952. DOI: 10.1016/j.gene.2019.143952. View

12.

Ye B, Liu B, Yang L, Zhu X, Zhang D, Wu W . controls self-renewal of embryonic stem cells via activating expression of transcription factor . EMBO J. 2018; 37(8). PMC: 5897780. DOI: 10.15252/embj.201797174. View

13.

Zhao W, Geng D, Li S, Chen Z, Sun M . LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer. Cancer Med. 2018; 7(3):842-855. PMC: 5852357. DOI: 10.1002/cam4.1353. View

14.

Xie X, Xu X, Sun C, Yu Z . Long intergenic noncoding RNA SNHG16 interacts with miR-195 to promote proliferation, invasion and tumorigenesis in hepatocellular carcinoma. Exp Cell Res. 2019; 383(1):111501. DOI: 10.1016/j.yexcr.2019.111501. View

15.

Niu J, Li X, Wang X, Liang C, Zhang Y, Li H . DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway. Cancer Cell Int. 2019; 19:168. PMC: 6591985. DOI: 10.1186/s12935-019-0883-1. View

16.

Li R, Zhu H, Yang D, Xia J, Zheng Z . Long noncoding RNA lncBRM promotes proliferation and invasion of colorectal cancer by sponging miR-204-3p and upregulating TPT1. Biochem Biophys Res Commun. 2018; 508(4):1259-1263. DOI: 10.1016/j.bbrc.2018.12.053. View

17.

Li O, Li Z, Tang Q, Li Y, Yuan S, Shen Y . Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 and MiR-4516. Med Sci Monit. 2018; 24:8510-8523. PMC: 6276722. DOI: 10.12659/MSM.911179. View

18.

Wang X, Guo G, Qian X, Dou D, Zhang Z, Xu X . miR-506 attenuates methylation of lncRNA to inhibit migration and invasion of breast cancer cell lines via targeting SP1 and SP3. Cancer Cell Int. 2018; 18:171. PMC: 6203274. DOI: 10.1186/s12935-018-0642-8. View

19.

Hu X, Wang J, He W, Zhao P, Wu W . Down-regulation of lncRNA Linc00152 suppressed cell viability, invasion, migration, and epithelial to mesenchymal transition, and reversed chemo-resistance in breast cancer cells. Eur Rev Med Pharmacol Sci. 2018; 22(10):3074-3084. DOI: 10.26355/eurrev_201805_15067. View

20.

Liu B, Ye B, Yang L, Zhu X, Huang G, Zhu P . Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression. Nat Immunol. 2017; 18(5):499-508. DOI: 10.1038/ni.3712. View