Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater

Overview

Journal Cell Rep Med

Publisher Cell Press

Specialties Biology
General Medicine

Date 2020 Sep 9

PMID 32904687

Citations 295

Authors

Artem Nemudryi

Anna Nemudraia

Tanner Wiegand

Kevin Surya

Murat Buyukyoruk

Calvin Cicha

Karl K Vanderwood

Royce Wilkinson

Blake Wiedenheft

Affiliations

Soon will be listed here.

Abstract

SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here, we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. In addition, we determine a nearly complete (98.5%) SARS-CoV-2 genome sequence from wastewater and use phylogenetic analysis to infer viral ancestry. Collectively, this work demonstrates how wastewater can be used as a proxy to monitor viral prevalence in the community and how genome sequencing can be used for genotyping viral strains circulating in a community.

Citing Articles

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