Identification of Pathways Associated with Placental Adaptation to Maternal Nutrient Restriction in Sheep

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2020 Sep 5

PMID 32887397

Citations 1

Authors

Ashley K Edwards

Kathrin A Dunlap

Thomas E Spencer

M Carey Satterfield

Affiliations

Soon will be listed here.

Abstract

Maternal nutrient restriction impairs placental growth and development, but available evidence suggests that adaptive mechanisms exist, in a subset of nutrient restricted (NR) ewes, that support normal fetal growth and do not result in intrauterine growth restriction (IUGR). This study utilized Affymetrix GeneChip Bovine and Ovine Genome 1.0 ST Arrays to identify novel placental genes associated with differential fetal growth rates within NR ewes. Singleton pregnancies were generated by embryo transfer and, beginning on Day 35 of pregnancy, ewes received either a 100% National Research Council (NRC) (control-fed group; = 7) or 50% NRC (NR group; = 24) diet until necropsy on Day 125. Fetuses from NR ewes were separated into NR non-IUGR ( = 6) and NR IUGR ( = 6) groups based on Day 125 fetal weight for microarray analysis. Of the 103 differentially expressed genes identified, 15 were upregulated and 88 were downregulated in NR non-IUGR compared to IUGR placentomes. Bioinformatics analysis revealed that upregulated gene clusters in NR non-IUGR placentomes associated with cell membranes, receptors, and signaling. Downregulated gene clusters associated with immune response, nutrient transport, and metabolism. Results illustrate that placentomal gene expression in late gestation is indicative of an altered placental immune response, which is associated with enhanced fetal growth, in a subpopulation of NR ewes.

Citing Articles

Placental Transcriptome Adaptations to Maternal Nutrient Restriction in Sheep.

Steinhauser C, Lambo C, Askelson K, Burns G, Behura S, Spencer T Int J Mol Sci. 2021; 22(14).

PMID: 34299281 PMC: 8306922. DOI: 10.3390/ijms22147654.

References

Owens J, Falconer J, Robinson J . Effect of restriction of placental growth on umbilical and uterine blood flows. Am J Physiol. 1986; 250(3 Pt 2):R427-34. DOI: 10.1152/ajpregu.1986.250.3.R427. View

Barker D . The origins of the developmental origins theory. J Intern Med. 2007; 261(5):412-7. DOI: 10.1111/j.1365-2796.2007.01809.x. View

Marsal K . Intrauterine growth restriction. Curr Opin Obstet Gynecol. 2002; 14(2):127-35. DOI: 10.1097/00001703-200204000-00005. View

Dunlap K, Brown J, Keith A, Satterfield M . Factors controlling nutrient availability to the developing fetus in ruminants. J Anim Sci Biotechnol. 2015; 6(1):16. PMC: 4407308. DOI: 10.1186/s40104-015-0012-5. View

Redmer D, Aitken R, Milne J, Reynolds L, Wallace J . Influence of maternal nutrition on messenger RNA expression of placental angiogenic factors and their receptors at midgestation in adolescent sheep. Biol Reprod. 2004; 72(4):1004-9. DOI: 10.1095/biolreprod.104.037234. View

Olsson M, Ekblom M, Fecker L, Kurkinen M, Ekblom P . cDNA cloning and embryonic expression of mouse nuclear pore membrane glycoprotein 210 mRNA. Kidney Int. 1999; 56(3):827-38. DOI: 10.1046/j.1523-1755.1999.00618.x. View

Yu Y, Singh U, Shi W, Konno T, Soares M, Geyer R . Influence of murine maternal diabetes on placental morphology, gene expression, and function. Arch Physiol Biochem. 2008; 114(2):99-110. DOI: 10.1080/13813450802033776. View

Riese R, Wolf P, Bromme D, Natkin L, Villadangos J, Ploegh H . Essential role for cathepsin S in MHC class II-associated invariant chain processing and peptide loading. Immunity. 1996; 4(4):357-66. DOI: 10.1016/s1074-7613(00)80249-6. View

Hahn-Zoric M, Hagberg H, Kjellmer I, Ellis J, Wennergren M, Hanson L . Aberrations in placental cytokine mRNA related to intrauterine growth retardation. Pediatr Res. 2002; 51(2):201-6. DOI: 10.1203/00006450-200202000-00013. View

10.

Satterfield M, Hayashi K, Song G, Black S, Bazer F, Spencer T . Progesterone regulates FGF10, MET, IGFBP1, and IGFBP3 in the endometrium of the ovine uterus. Biol Reprod. 2008; 79(6):1226-36. DOI: 10.1095/biolreprod.108.071787. View

11.

Rogge L, Biffi M, Passini N, Presky D, Gubler U, Sinigaglia F . Selective expression of an interleukin-12 receptor component by human T helper 1 cells. J Exp Med. 1997; 185(5):825-31. PMC: 2196163. DOI: 10.1084/jem.185.5.825. View

12.

Rogge L, Papi A, Presky D, Biffi M, Minetti L, Miotto D . Antibodies to the IL-12 receptor beta 2 chain mark human Th1 but not Th2 cells in vitro and in vivo. J Immunol. 1999; 162(7):3926-32. View

13.

Bertucci M, Loose J, Wallace E, Jenkin G, Miller S . Anti-inflammatory therapy in an ovine model of fetal hypoxia induced by single umbilical artery ligation. Reprod Fertil Dev. 2011; 23(2):346-52. DOI: 10.1071/RD10110. View

14.

Ma Y, Zhu M, Uthlaut A, Nijland M, Nathanielsz P, Hess B . Upregulation of growth signaling and nutrient transporters in cotyledons of early to mid-gestational nutrient restricted ewes. Placenta. 2011; 32(3):255-63. PMC: 3049449. DOI: 10.1016/j.placenta.2011.01.007. View

15.

Streyl D, Kenngott R, Herbach N, Wanke R, Blum H, Sinowatz F . Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes. Reproduction. 2011; 143(1):85-105. DOI: 10.1530/REP-11-0204. View

16.

Zeisel S . Choline: critical role during fetal development and dietary requirements in adults. Annu Rev Nutr. 2006; 26:229-50. PMC: 2441939. DOI: 10.1146/annurev.nutr.26.061505.111156. View

17.

Spencer T, Forde N, Dorniak P, Hansen T, Romero J, Lonergan P . Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants. Reproduction. 2013; 146(4):377-87. PMC: 3791335. DOI: 10.1530/REP-13-0165. View

18.

Cross J, Mickelson L . Nutritional influences on implantation and placental development. Nutr Rev. 2006; 64(5 Pt 2):S12-8. DOI: 10.1301/nr.may.s12-s18. View

19.

Reynolds L, Redmer D . Angiogenesis in the placenta. Biol Reprod. 2001; 64(4):1033-40. DOI: 10.1095/biolreprod64.4.1033. View

20.

Huang D, Sherman B, Lempicki R . Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009; 4(1):44-57. DOI: 10.1038/nprot.2008.211. View