Cellular Responses to Deproteinized Bovine Bone Mineral Biofunctionalized with Bone-conditioned Medium

Overview

Journal Clin Oral Investig

Specialty Dentistry

Date 2020 Sep 2

PMID 32870390

Citations 8

Authors

Ludovica Parisi

Daniel Buser

Vivianne Chappuis

Maria B Asparuhova

Affiliations

Soon will be listed here.

Abstract

Objectives: The aim of the study was to investigate whether the osteoinductive properties of bone-conditioned medium (BCM) harvested from cortical bone chips within a clinically relevant short-term period can enhance the biologic characteristics of deproteinized bovine bone mineral (DBBM) in vitro.

Materials And Methods: To assess the biofunctionalization of DBBM, the adhesive, proliferative, and differentiation properties of mesenchymal stromal ST2, pre-osteoblastic MC3T3-E1, and primary bone-derived cells grown on BCM-coated DBBM were examined by crystal violet staining of adherent cells, BrdU ELISA, and qRT-PCR, respectively.

Results: BCM extracted within 20 min or 24 h in either Ringer's solution (BCM-RS) or RS mixed with autologous serum (BCM-RS + S) increased the adhesive properties of all three cell types seeded on DBBM. The 20-min BCM-RS preparation appeared more potent than the 24-h preparation. BCM-RS made within 20 min or 24 h had strong pro-proliferative effects on all cell types grown on DBBM. RS + S alone exhibited a considerable pro-proliferative effect, suggesting an impact of the serum on cellular growth. DBBM coated with BCM-RS or BCM-RS + S, made within 20 min or 24 h each, caused a significant induction of osteogenic differentiation marker expression with a higher potency of the BCM-RS + S. Finally, a strong additive effect of fresh bone chips combined with BCM-coated DBBM on the osteogenic differentiation of the three cell types was observed.

Conclusions: Altogether, the data strongly support the biofunctionalization of DBBM with BCM extracted within a clinically relevant time window of 20 min.

Clinical Relevance: Pre-activation of non-osteoinductive biomaterials with BCM, prepared from autologous bone chips during a guided bone regeneration (GBR) procedure, bears the potential of an optimal treatment modality for bone defects in daily practice.

Citing Articles

Biofunctionalization of Collagen Barrier Membranes with Bone-Conditioned Medium, as a Natural Source of Growth Factors, Enhances Osteoblastic Cell Behavior.

Ashoka Sreeja H, Couso-Queiruga E, Raabe C, Chappuis V, Asparuhova M Int J Mol Sci. 2025; 26(4).

PMID: 40004074 PMC: 11855076. DOI: 10.3390/ijms26041610.

Use of Deproteinized Bovine Bone in Association with Calcium Sulphate for Alveolar Socket Preservation.

Signoriello A, Zangani A, Faccioni P, Messina E, Pardo A, Corrocher G J Clin Med. 2025; 14(1.

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Horizontal Bone Augmentation with Simultaneous Implant Placement in the Aesthetic Region: A Case Report and Review of the Current Evidence.

Izzetti R, Rossana I, Cinquini C, Chiara C, Fortunato A, Alfonsi F Medicina (Kaunas). 2024; 60(11.

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Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface .

Parisi L, Bianchi M, Ghezzi B, Maurizi E, Macaluso G, Bussolati O J Dent Sci. 2023; 18(4):1630-1637.

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Effect of three different grafting materials on immediate implant placement using vestibular socket therapy in class II extraction sockets in the maxillary esthetic zone: a randomized controlled clinical trial.

Hamed M, El-Tonsy M, Elaskary A, Abdelaziz G, Saeed S, Elfahl B BMC Oral Health. 2023; 23(1):623.

PMID: 37658342 PMC: 10474704. DOI: 10.1186/s12903-023-03345-9.

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