Eighteen New Aeruginosamide Variants Produced by the Baltic Cyanobacterium CCNP1324

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2020 Sep 2

PMID 32867236

Citations 5

Authors

Marta Ceglowska

Karolia Szubert

Ewa Wieczerzak

Alicja Kosakowska

Hanna Mazur-Marzec

Affiliations

Soon will be listed here.

Abstract

Cyanobactins are a large family of ribosomally synthesized and post-translationally modified cyanopeptides (RiPPs). Thus far, over a hundred cyanobactins have been detected in different free-living and symbiotic cyanobacteria. The majority of these peptides have a cyclic structure. The occurrence of linear cyanobactins, aeruginosamides and virenamide, has been reported sporadically and in few cyanobacterial taxa. In the current work, the production of cyanobactins by sp. CCNP1324, isolated from the brackish water Baltic Sea, has been studied for the first time. In the strain, eighteen new aeruginosamide (AEG) variants have been detected. These compounds are characterized by the presence of prenyl and thiazole groups. A common element of AEGs produced by sp. CCNP1324 is the sequence of the three C-terminal residues containing proline, pyrrolidine and methyl ester of thiazolidyne-4-carboxylic acid (Pro-Pyr-TzlCOOMe) or thiazolidyne-4-carboxylic acid (Pro-Pyr-TzlCOOH). The aeruginosamides with methylhomotyrosine (MeHTyr) and with the unidentified -terminal amino acids showed strong cytotoxic activity against human breast cancer cells (T47D).

Citing Articles

Recent Advances in Discovery, Bioengineering, and Bioactivity-Evaluation of Ribosomally Synthesized and Post-translationally Modified Peptides.

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Biological Activity and Stability of Aeruginosamides from Cyanobacteria.

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