Automated High-content Imaging for Cellular Uptake, from the Schmuck Cation to the Latest Cyclic Oligochalcogenides

Overview

Journal Beilstein J Org Chem

Specialty Chemistry

Date 2020 Aug 25

PMID 32831957

Citations 4

Authors

Remi Martinent

Javier Lopez-Andarias

Dimitri Moreau

Yangyang Cheng

Naomi Sakai

Stefan Matile

Affiliations

Soon will be listed here.

Abstract

Recent progress with chemistry tools to deliver into living cells has seen a shift of attention from counterion-mediated uptake of cell-penetrating peptides (CPPs) and their mimics, particularly the Schmuck cation, toward thiol-mediated uptake with cell-penetrating poly(disulfide)s (CPDs) and cyclic oligochalcogenides (COCs), here exemplified by asparagusic acid. A persistent challenge in this evolution is the simultaneous and quantitative detection of cytosolic delivery and cytotoxicity in a high-throughput format. Here, we show that the combination of the HaloTag-based chloroalkane penetration assay (CAPA) with automated high-content (HC) microscopy can satisfy this need. The automated imaging of thousands of cells per condition in multiwell plates allows us to obtain quantitative data on not only the fluorescence intensity but also on the localization in a very short time. Quantitative and statistically relevant results can be obtained from dose-response curves of the targeted delivery to selected cells and the cytotoxicity in the same experiment, even with poorly optimized cellular systems.

Citing Articles

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Inhibitors of thiol-mediated uptake.

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