LncRNA TUG1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma Via Regulating MiR-29c-3p/ Axis

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Aug 22

PMID 32821161

Citations 11

Authors

Wei Zhao

Xue Jiang

Shuxia Yang

Affiliations

Soon will be listed here.

Abstract

Background: Taurine upregulated gene 1 (TUG1) has been recognized as a novel oncogenic gene. The current study was established to explore the function and regulatory mechanism of TUG1 in hepatocellular carcinoma (HCC).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of TUG1, miR-29c-3p, and in tissues and cell lines. MTT assay, wound-healing and transwell assay were utilized for the detection of cell viability, migration and invasion, respectively. The interactions between miR-29c-3p and TUG1/ were predicted by starBase v2.0 (http://starbase.sysu.edu.cn/) and verified by the dual-luciferase reporter or RNA immunoprecipitation assay. Western blot assay was performed to determine the protein levels of COL1A1, cyclin D1, E-cadherin, N-cadherin, Bcl-2, and Bax.

Results: Dramatically increased expression of TUG1 was noticed in HCC tissues and cell lines. TUG1 knockdown restrained the proliferation, migration, and invasion, and promoted the apoptosis of HCC cells. TUG1 targeted miR-29c-3p and inhibited miR-29c-3p expression. Overexpression of miR-29c-3p inhibited the proliferation, migration and invasion of HCC cells. MiR-29c-3p directly targeted and down-regulated expression. In addition, downregulation of miR-29c-3p and upregulation of both reversed the effects of TUG1 knockdown on the proliferation, apoptosis, migration, and invasion of HCC cells.

Conclusion: TUG1 could promote the proliferation, migration and invasion of HCC cells through regulating miR-29c-3p/ axis. This novel finding might provide a latent target for the treatment of HCC.

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