-dependent Coordination of Mouse Cardiac Progenitor Cell Patterning and Differentiation

Overview

Journal Elife

Specialty Biology

Date 2020 Aug 18

PMID 32804075

Citations 30

Authors

Sonia Stefanovic

Brigitte Laforest

Jean-Pierre Desvignes

Fabienne Lescroart

Laurent Argiro

Corinne Maurel-Zaffran

David Salgado

Elise Plaindoux

Christopher De Bono

Kristijan Pazur

Magali Theveniau-Ruissy

Christophe Beroud

Michel Puceat

Anthony Gavalas

Robert G Kelly

Stephane Zaffran

Affiliations

Soon will be listed here.

Abstract

Perturbation of addition of second heart field (SHF) cardiac progenitor cells to the poles of the heart tube results in congenital heart defects (CHD). The transcriptional programs and upstream regulatory events operating in different subpopulations of the SHF remain unclear. Here, we profile the transcriptome and chromatin accessibility of anterior and posterior SHF sub-populations at genome-wide levels and demonstrate that Hoxb1 negatively regulates differentiation in the posterior SHF. Spatial mis-expression of in the anterior SHF results in hypoplastic right ventricle. Activation of in embryonic stem cells arrests cardiac differentiation, whereas -deficient mouse embryos display premature cardiac differentiation. Moreover, ectopic differentiation in the posterior SHF of embryos lacking both and its paralog results in atrioventricular septal defects. Our results show that Hoxb1 plays a key role in patterning cardiac progenitor cells that contribute to both cardiac poles and provide new insights into the pathogenesis of CHD.

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