The Role of LncRNA in Squamous Cell Carcinoma of the Head and Neck is Still Difficult to Define

Overview

Journal Contemp Oncol (Pozn)

Publisher Termedia

Specialty Oncology

Date 2020 Aug 11

PMID 32774134

Citations 5

Authors

Joanna Kozlowska

Kinga Koziol

Maciej Stasiak

Justyna Obacz

Kacper Guglas

Paulina Poter

Andrzej Mackiewicz

Tomasz Kolenda

Affiliations

Soon will be listed here.

Abstract

Introduction: Nuclear paraspeckle assembly transcript 1 () is considered an oncogene in various cancers, but the role in head and neck squamous cell carcinomas (HNSCC) is not clear.

Material And Methods: Expression of in HNSCC patients' samples and cell lines was analysed using qRT-PCR. The TCGA expression data of were analysed depending on the clinicopathological parameters and tumour localisation. Correlation and gene set enrichment analysis (GSEA) were conducted, and the results were analysed using the REACTOME and GeneMANIA tools. All statistical analyses were carried out using GraphPad Prism 5 and Statistica 13.

Results: The was up-regulated in some patients' samples and HNSCC cell lines. Moreover, TCGA data analysis indicated that the expression of was up-regulated in tumour tissue in most of the analysed TCGA cancers, including HNSCC. There were no significant differences in levels of between various tumour localisations. Overall survival of individuals with high expression of was slightly longer than in the low-expression group ( = 0.0553). Analysis of genes that positively and negatively correlated with indicated that they are involved in mRNA metabolism and cellular transport. Moreover, the GSEA revealed that in patients with low , the most up-regulated genes were in clusters associated with the cAMP-dependent pathway, the MYC pathway, unfolded protein response, the MTORC1 signalling pathway, oxidative phosphorylation, and DNA repair.

Conclusions: Patients with low expression of display worse overall survival, presumably due to up-regulation of certain oncogenic signalling pathways that are important for cancerogenesis.

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