The Long Non-coding RNA NEAT1 Regulates Epithelial to Mesenchymal Transition and Radioresistance in Through MiR-204/ZEB1 Axis in Nasopharyngeal Carcinoma

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2016 Mar 30

PMID 27020592

Citations 89

Authors

Yaoyong Lu

Tao Li

Ganbao Wei

Liangbo Liu

Qinsheng Chen

Lufei Xu

Kunqiang Zhang

Dehao Zeng

Rongwei Liao

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) play a critical role in cancer progression, including in nasopharyngeal carcinoma (NPC). However, it is still poorly understood whether lncRNA regulates epithelial to mesenchymal transition (EMT) and radioresistance of NPC cells. We found that lncRNA NEAT1 was significantly upregulated in NPC cell lines and tissues. Knockdown of NEAT1 could sensitize NPC cells to radiation in vitro. Further investigation found that NEAT1 regulated radioresistance by modulating EMT phenotype. Furthermore, we found that there was reciprocal repression between NEAT1 and miR-204. ZEB1 was identified as a downstream target of miR-204 and NEAT1 upregulated ZEB1 expression by negatively regulating miR-204 expression. Taking together, we proposed that NEAT1 regulated EMT phenotype and radioresistance by modulating the miR-204/ZEB1 axis in NPC.

Citing Articles

A fishnet between nasopharyngeal carcinoma and resistance: the competing endogenous RNA network.

Li M, Mo T, Yang Z, Yao Y, Wang Y, Zhao Y Curr Treat Options Oncol. 2025; 26(1):45-54.

PMID: 39810047 DOI: 10.1007/s11864-024-01284-3.

Radioresistance and brain metastases: a review of the literature and applied perspective.

Youssef A, Sahgal A, Das S Front Oncol. 2024; 14:1477448.

PMID: 39540151 PMC: 11557554. DOI: 10.3389/fonc.2024.1477448.

The association between the expression level of nuclear paraspeckle assembly transcript 1 and the survival rate of head and neck cancer patients after treatment.

Lin N, Hsia S, Vu Nguyen T, Wang T, Sun K, Chiu K J Dent Sci. 2024; 19(4):2074-2081.

PMID: 39347098 PMC: 11437243. DOI: 10.1016/j.jds.2024.05.001.

Role of Non-coding RNAs on the Radiotherapy Sensitivity and Resistance in Cancer Cells.

Jalali-Zefrei F, Mousavi S, Delpasand K, Shourmij M, Farzipour S Curr Gene Ther. 2024; 25(2):113-135.

PMID: 38676526 DOI: 10.2174/0115665232301727240422092311.

Hypoxia Induces Tumor-Derived Exosome SNHG16 to Mediate Nasopharyngeal Carcinoma Progression through the miR-23b-5p/MCM6 Pathway.

Hou W, Xu L, Su T, Wu Y, Liu Y, Wei Y Appl Biochem Biotechnol. 2023; 196(1):275-295.

PMID: 37119503 DOI: 10.1007/s12010-023-04558-y.

References

Deng X, Ma L, Wu M, Zhang G, Jin C, Guo Y . miR-124 radiosensitizes human glioma cells by targeting CDK4. J Neurooncol. 2013; 114(3):263-74. DOI: 10.1007/s11060-013-1179-2. View

Wilusz J, Sunwoo H, Spector D . Long noncoding RNAs: functional surprises from the RNA world. Genes Dev. 2009; 23(13):1494-504. PMC: 3152381. DOI: 10.1101/gad.1800909. View

Gregory R, Chendrimada T, Cooch N, Shiekhattar R . Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell. 2005; 123(4):631-40. DOI: 10.1016/j.cell.2005.10.022. View

Chiyomaru T, Fukuhara S, Saini S, Majid S, Deng G, Shahryari V . Long non-coding RNA HOTAIR is targeted and regulated by miR-141 in human cancer cells. J Biol Chem. 2014; 289(18):12550-65. PMC: 4007447. DOI: 10.1074/jbc.M113.488593. View

Chang L, Graham P, Hao J, Ni J, Bucci J, Cozzi P . Acquisition of epithelial-mesenchymal transition and cancer stem cell phenotypes is associated with activation of the PI3K/Akt/mTOR pathway in prostate cancer radioresistance. Cell Death Dis. 2013; 4:e875. PMC: 3920940. DOI: 10.1038/cddis.2013.407. View

Ma L, Deng X, Wu M, Zhang G, Huang J . Down-regulation of miRNA-204 by LMP-1 enhances CDC42 activity and facilitates invasion of EBV-associated nasopharyngeal carcinoma cells. FEBS Lett. 2014; 588(9):1562-70. DOI: 10.1016/j.febslet.2014.02.039. View

Su H, Jin X, Shen L, Fang Y, Fei Z, Zhang X . Inhibition of cyclin D1 enhances sensitivity to radiotherapy and reverses epithelial to mesenchymal transition for esophageal cancer cells. Tumour Biol. 2015; 37(4):5355-63. DOI: 10.1007/s13277-015-4393-z. View

Lee A, Poon Y, Foo W, Law S, Cheung F, Chan D . Retrospective analysis of 5037 patients with nasopharyngeal carcinoma treated during 1976-1985: overall survival and patterns of failure. Int J Radiat Oncol Biol Phys. 1992; 23(2):261-70. DOI: 10.1016/0360-3016(92)90740-9. View

Nakagawa S, Shimada M, Yanaka K, Mito M, Arai T, Takahashi E . The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice. Development. 2014; 141(23):4618-27. PMC: 4302932. DOI: 10.1242/dev.110544. View

10.

Chen L, Zhao J . Functional analysis of long noncoding RNAs in development and disease. Adv Exp Med Biol. 2014; 825:129-58. DOI: 10.1007/978-1-4939-1221-6_4. View

11.

Teoh J, Yunus R, Hassan F, Ghazali N, Abidin Z . Nasopharyngeal carcinoma in dermatomyositis patients: A 10-year retrospective review in Hospital Selayang, Malaysia. Rep Pract Oncol Radiother. 2014; 19(5):332-6. PMC: 4150095. DOI: 10.1016/j.rpor.2014.02.005. View

12.

Johnsson P, Morris K . Expanding the functional role of long noncoding RNAs. Cell Res. 2014; 24(11):1284-5. PMC: 4220152. DOI: 10.1038/cr.2014.104. View

13.

Wei W, Sham J . Nasopharyngeal carcinoma. Lancet. 2005; 365(9476):2041-54. DOI: 10.1016/S0140-6736(05)66698-6. View

14.

Zhang L, Wang X, Chen P . MiR-204 down regulates SIRT1 and reverts SIRT1-induced epithelial-mesenchymal transition, anoikis resistance and invasion in gastric cancer cells. BMC Cancer. 2013; 13:290. PMC: 3710153. DOI: 10.1186/1471-2407-13-290. View

15.

Jalali S, Bhartiya D, Lalwani M, Sivasubbu S, Scaria V . Systematic transcriptome wide analysis of lncRNA-miRNA interactions. PLoS One. 2013; 8(2):e53823. PMC: 3566149. DOI: 10.1371/journal.pone.0053823. View

16.

Zeng J, Wei M, Shi R, Cai C, Liu X, Li T . MiR-204-5p/Six1 feedback loop promotes epithelial-mesenchymal transition in breast cancer. Tumour Biol. 2015; 37(2):2729-35. DOI: 10.1007/s13277-015-4039-1. View

17.

Kang J, Kim E, Kim W, Seong K, Youn H, Kim J . Rhamnetin and cirsiliol induce radiosensitization and inhibition of epithelial-mesenchymal transition (EMT) by miR-34a-mediated suppression of Notch-1 expression in non-small cell lung cancer cell lines. J Biol Chem. 2013; 288(38):27343-27357. PMC: 3779729. DOI: 10.1074/jbc.M113.490482. View

18.

Preca B, Bajdak K, Mock K, Sundararajan V, Pfannstiel J, Maurer J . A self-enforcing CD44s/ZEB1 feedback loop maintains EMT and stemness properties in cancer cells. Int J Cancer. 2015; 137(11):2566-77. DOI: 10.1002/ijc.29642. View

19.

Karreth F, Pandolfi P . ceRNA cross-talk in cancer: when ce-bling rivalries go awry. Cancer Discov. 2013; 3(10):1113-21. PMC: 3801300. DOI: 10.1158/2159-8290.CD-13-0202. View

20.

Su H, Jin X, Zhang X, Zhao L, Lin B, Li L . FH535 increases the radiosensitivity and reverses epithelial-to-mesenchymal transition of radioresistant esophageal cancer cell line KYSE-150R. J Transl Med. 2015; 13:104. PMC: 4384308. DOI: 10.1186/s12967-015-0464-6. View