Rapid Genotyping Protocol to Improve Dengue Virus Serotype 2 Survey in Lao PDR

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Aug 9

PMID 32764809

Citations 4

Authors

Elodie Calvez

Somphavanh Somlor

Souksakhone Viengphouthong

Charlotte Baliere

Phaithong Bounmany

Sitsana Keosenhom

Valerie Caro

Marc Grandadam

Affiliations

Soon will be listed here.

Abstract

Dengue fever is one of the major public health problems in Lao PDR. Over the last decade, dengue virus (DENV) epidemics were characterized by a novel predominant serotype accompanied by at least two other serotypes. Since 2008, DENV-2 circulated at a low level in Lao PDR but its epidemiologic profile changed at the end of 2018. Indeed, the number of confirmed DENV-2 cases suddenly increased in October 2018 and DENV-2 became predominant at the country level in early 2019. We developed a Genotype Screening Protocol (GSP) to determine the origin(s) of the Lao DENV-2 and study their genetic polymorphism. With a good correlation with full envelope gene sequencing data, this molecular epidemiology tool evidence the co-circulation of two highly polymorphic DENV-2 genotypes, i.e. Asian I and Cosmopolitan genotypes, over the last five years, suggesting multiple introductions of DENV-2 in the country. GSP approach provides relevant first line information that may help countries with limited laboratory resources to reinforce their capabilities to DENV-2 and to follow the epidemics progresses and assess situations at the regional level.

Citing Articles

Genomic Characterization of Circulating Dengue Virus, Ethiopia, 2022-2023.

Abera A, Tegally H, Tasew G, Wilkinson E, Ali A, Regasa F Emerg Infect Dis. 2025; 31(3):516-525.

PMID: 40023801 PMC: 11878302. DOI: 10.3201/eid3103.240996.

Molecular Epidemiology of Dengue Viruses in Lao People's Democratic Republic, 2020-2023.

Troupin C, Intavong K, Somlor S, Viengphouthong S, Keosenhom S, Chindavong T Microorganisms. 2025; 13(2).

PMID: 40005687 PMC: 11857872. DOI: 10.3390/microorganisms13020318.

A Six Years (2010-2016) Longitudinal Survey of the Four Serotypes of Dengue Viruses in Lao PDR.

Baliere C, Calvez E, Thiberge J, Somlor S, Vandenbogaert M, Grandadam M Microorganisms. 2023; 11(2).

PMID: 36838207 PMC: 9959689. DOI: 10.3390/microorganisms11020243.

Multiple chikungunya virus introductions in Lao PDR from 2014 to 2020.

Calvez E, Bounmany P, Somlor S, Xaybounsou T, Viengphouthong S, Keosenhom S PLoS One. 2022; 17(7):e0271439.

PMID: 35839218 PMC: 9286254. DOI: 10.1371/journal.pone.0271439.

Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR.

Calvez E, Bounmany P, Baliere C, Somlor S, Viengphouthong S, Xaybounsou T Microorganisms. 2021; 9(11).

PMID: 34835389 PMC: 8617722. DOI: 10.3390/microorganisms9112263.

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