Flow Cytometric Identification of T13 Cells in Mouse and Human

Overview

Journal J Allergy Clin Immunol

Specialty Allergy & Immunology

Date 2020 Jul 26

PMID 32709424

Citations 7

Authors

Jennifer S Chen

Jessica D S Grassmann

Uthaman Gowthaman

Sam J Olyha

Tregony Simoneau

M Cecilia Berin

Stephanie C Eisenbarth

Adam Williams

Affiliations

Soon will be listed here.

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T13 cells in both mice and humans.

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