HDX-MS: An Analytical Tool to Capture Protein Motion in Action

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2020 Jul 26

PMID 32709043

Citations 16

Authors

Dominic Narang

Cristina Lento

Derek J Wilson

Affiliations

Soon will be listed here.

Abstract

Virtually all protein functions in the cell, including pathogenic processes, require coordinated motion of atoms or domains, i.e., conformational dynamics. Understanding protein dynamics is therefore critical both for drug development and to learn about the underlying molecular causes of many diseases. Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) provides valuable information about protein dynamics, which is highly complementary to the static picture provided by conventional high-resolution structural tools (i.e., X-ray crystallography and structural NMR). The amount of protein required to carry out HDX-MS experiments is a fraction of the amount required by alternative biophysical techniques, which are also usually lower resolution. Use of HDX-MS is growing quickly both in industry and academia, and it has been successfully used in numerous drug and vaccine development efforts, with important roles in understanding allosteric effects and mapping binding sites.

Citing Articles

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