Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner Using TMT10plex Mass Tag Labeling

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2020 Jul 21

PMID 32685930

Citations 6

Authors

Andrew W Jones

Helen R Flynn

Frank Uhlmann

Ambrosius P Snijders

Sandra A Touati

Affiliations

Soon will be listed here.

Abstract

Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).

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