Mediated Defense Responses in Grapevine Offspring Resistant to

Overview

Journal Plants (Basel)

Date 2020 Jun 26

PMID 32580445

Citations 12

Authors

Tyrone Possamai

Daniele Migliaro

Massimo Gardiman

Riccardo Velasco

Barbara De Nardi

Affiliations

Soon will be listed here.

Abstract

Downy mildew, caused by the biotrophic oomycete , is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-assisted-selection (MAS) provide fast and cost-effective genotyping methods, but phenotyping remains necessary to characterize the host-pathogen interaction and assess the effective resistance level of new varieties as well as to validate MAS selection. In this study, the mediated defense responses were investigated in 31 genotypes, encompassing susceptible and resistant varieties and 26 seedlings, following inoculation of leaf discs with . The offspring differed in loci inherited (none, one or two): and from Solaris and and from Kozma 20-3. To improve the assessment of different resistance responses, pathogen reaction (sporulation) and host reaction (necrosis) were scored separately as independent features. They were differently expressed depending on locus: offspring carrying and loci showed the strongest resistance response (scarce sporulation and necrosis), those carrying locus showed the highest levels of necrosis while carrying genotypes showed intermediate levels of both sporulation and necrosis.

Citing Articles

: A Haplotype Variant of Locus Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine.

Hoschele T, Malagol N, Bori S, Mullner S, Topfer R, Sturm J Plants (Basel). 2024; 13(18).

PMID: 39339604 PMC: 11434656. DOI: 10.3390/plants13182624.

Genes Introgressed with RUN1 and RPV1: Poor Recombination and Impact on Berry Transcriptome.

Shi M, Savoi S, Sarah G, Soriano A, Weber A, Torregrosa L Plants (Basel). 2024; 13(15).

PMID: 39124212 PMC: 11314213. DOI: 10.3390/plants13152095.

A Sustainable Strategy for Marker-Assisted Selection (MAS) Applied in Grapevine ( spp.) Breeding for Resistance to Downy () and Powdery () Mildews.

Possamai T, Scota L, Velasco R, Migliaro D Plants (Basel). 2024; 13(14).

PMID: 39065527 PMC: 11280485. DOI: 10.3390/plants13142001.

Phenotyping grapevine resistance to downy mildew: deep learning as a promising tool to assess sporulation and necrosis.

Macia F, Possamai T, Dorne M, Lacombe M, Duchene E, Merdinoglu D Plant Methods. 2024; 20(1):90.

PMID: 38872155 PMC: 11177444. DOI: 10.1186/s13007-024-01220-4.

Phased grapevine genome sequence of an carrier for biotechnological exploration of resistance to .

Frommer B, Mullner S, Holtgrawe D, Viehover P, Huettel B, Topfer R Front Plant Sci. 2023; 14:1180982.

PMID: 37223784 PMC: 10200900. DOI: 10.3389/fpls.2023.1180982.

References

Venuti S, Copetti D, Foria S, Falginella L, Hoffmann S, Bellin D . Historical introgression of the downy mildew resistance gene Rpv12 from the Asian species Vitis amurensis into grapevine varieties. PLoS One. 2013; 8(4):e61228. PMC: 3625174. DOI: 10.1371/journal.pone.0061228. View

Diez-Navajas A, Wiedemann-Merdinoglu S, Greif C, Merdinoglu D . Nonhost versus host resistance to the grapevine downy mildew, Plasmopara viticola, studied at the tissue level. Phytopathology. 2008; 98(7):776-80. DOI: 10.1094/PHYTO-98-7-0776. View

Delmotte F, Mestre P, Schneider C, Kassemeyer H, Kozma P, Richart-Cervera S . Rapid and multiregional adaptation to host partial resistance in a plant pathogenic oomycete: evidence from European populations of Plasmopara viticola, the causal agent of grapevine downy mildew. Infect Genet Evol. 2013; 27:500-8. DOI: 10.1016/j.meegid.2013.10.017. View

Di Gaspero G, Copetti D, Coleman C, Castellarin S, Eibach R, Kozma P . Selective sweep at the Rpv3 locus during grapevine breeding for downy mildew resistance. Theor Appl Genet. 2011; 124(2):277-86. DOI: 10.1007/s00122-011-1703-8. View

Vezzulli S, Malacarne G, Masuero D, Vecchione A, Dolzani C, Goremykin V . The Haplotype and Stilbenoid Induction Mediate Downy Mildew Resistance in a Grapevine Interspecific Population. Front Plant Sci. 2019; 10:234. PMC: 6414455. DOI: 10.3389/fpls.2019.00234. View

Bellin D, Peressotti E, Merdinoglu D, Wiedemann-Merdinoglu S, Adam-Blondon A, Cipriani G . Resistance to Plasmopara viticola in grapevine 'Bianca' is controlled by a major dominant gene causing localised necrosis at the infection site. Theor Appl Genet. 2009; 120(1):163-76. DOI: 10.1007/s00122-009-1167-2. View

Blasi P, Blanc S, Wiedemann-Merdinoglu S, Prado E, Ruhl E, Mestre P . Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew. Theor Appl Genet. 2011; 123(1):43-53. DOI: 10.1007/s00122-011-1565-0. View

Cadle-Davidson L . Variation Within and Between Vitis spp. for Foliar Resistance to the Downy Mildew Pathogen Plasmopara viticola. Plant Dis. 2019; 92(11):1577-1584. DOI: 10.1094/PDIS-92-11-1577. View

Fischer B, Salakhutdinov I, Akkurt M, Eibach R, Edwards K, Topfer R . Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theor Appl Genet. 2003; 108(3):501-15. DOI: 10.1007/s00122-003-1445-3. View

10.

Divilov K, Barba P, Cadle-Davidson L, Reisch B . Single and multiple phenotype QTL analyses of downy mildew resistance in interspecific grapevines. Theor Appl Genet. 2018; 131(5):1133-1143. PMC: 5895686. DOI: 10.1007/s00122-018-3065-y. View

11.

Oerke E, Herzog K, Toepfer R . Hyperspectral phenotyping of the reaction of grapevine genotypes to Plasmopara viticola. J Exp Bot. 2016; 67(18):5529-5543. DOI: 10.1093/jxb/erw318. View

12.

Schwander F, Eibach R, Fechter I, Hausmann L, Zyprian E, Topfer R . Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine. Theor Appl Genet. 2011; 124(1):163-76. DOI: 10.1007/s00122-011-1695-4. View