The Haplotype and Stilbenoid Induction Mediate Downy Mildew Resistance in a Grapevine Interspecific Population

Overview

Journal Front Plant Sci

Date 2019 Mar 22

PMID 30894868

Citations 30

Authors

Silvia Vezzulli

Giulia Malacarne

Domenico Masuero

Antonella Vecchione

Chiara Dolzani

Vadim Goremykin

Zeraye Haile Mehari

Elisa Banchi

Riccardo Velasco

Marco Stefanini

Urska Vrhovsek

Luca Zulini

Pietro Franceschi

Claudio Moser

Affiliations

Soon will be listed here.

Abstract

The development of new resistant varieties to the oomycete (Berk.& Curt) is a promising way to combat downy mildew (DM), one of the major diseases threatening the cultivated grapevine ( L.). Taking advantage of a segregating population derived from "Merzling" (a mid-resistant hybrid) and "Teroldego" (a susceptible landrace), 136 F1 individuals were characterized by combining genetic, phenotypic, and gene expression data to elucidate the genetic basis of DM resistance and polyphenol biosynthesis upon infection. An improved consensus linkage map was obtained by scoring 192 microsatellite markers. The progeny were screened for DM resistance and production of 42 polyphenols. QTL mapping showed that DM resistance is associated with the herein named specific haplotype and it identified 46 novel metabolic QTLs linked to 30 phenolics-related parameters. A list of the 95 most relevant candidate genes was generated by specifically exploring the stilbenoid-associated QTLs. Expression analysis of 11 genes in genotypes displaying disparity in DM resistance level and stilbenoid accumulation revealed significant new candidates for the genetic control of stilbenoid biosynthesis and oligomerization. These overall findings emphasized that DM resistance is likely mediated by the major haplotype and stilbenoid induction.

Citing Articles

: A Haplotype Variant of Locus Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine.

Hoschele T, Malagol N, Bori S, Mullner S, Topfer R, Sturm J Plants (Basel). 2024; 13(18).

PMID: 39339604 PMC: 11434656. DOI: 10.3390/plants13182624.

The Transcriptional Landscape of Berry Skin in Red and White PIWI ("Pilzwiderstandsfähig") Grapevines Possessing QTLs for Partial Resistance to Downy and Powdery Mildews.

Scariolo F, Gabelli G, Magon G, Palumbo F, Pirrello C, Farinati S Plants (Basel). 2024; 13(18).

PMID: 39339549 PMC: 11434962. DOI: 10.3390/plants13182574.

A Sustainable Strategy for Marker-Assisted Selection (MAS) Applied in Grapevine ( spp.) Breeding for Resistance to Downy () and Powdery () Mildews.

Possamai T, Scota L, Velasco R, Migliaro D Plants (Basel). 2024; 13(14).

PMID: 39065527 PMC: 11280485. DOI: 10.3390/plants13142001.

Basic leucine zipper gene is expressed at a quantitative trait locus for high monoterpene content in grape berries.

Zhang Y, Liu C, Liu X, Wang Z, Wang Y, Zhong G Hortic Res. 2023; 10(9):uhad151.

PMID: 37701455 PMC: 10493639. DOI: 10.1093/hr/uhad151.

Fungicide-Saving Potential and Economic Advantages of Fungus-Resistant Grapevine Cultivars.

Eisenmann B, Wingerter C, Dressler M, Freund C, Kortekamp A, Bogs J Plants (Basel). 2023; 12(17).

PMID: 37687364 PMC: 10489737. DOI: 10.3390/plants12173120.

References

Jaillon O, Aury J, Noel B, Policriti A, Clepet C, Casagrande A . The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature. 2007; 449(7161):463-7. DOI: 10.1038/nature06148. View

Vitulo N, Forcato C, Carpinelli E, Telatin A, Campagna D, DAngelo M . A deep survey of alternative splicing in grape reveals changes in the splicing machinery related to tissue, stress condition and genotype. BMC Plant Biol. 2014; 14:99. PMC: 4108029. DOI: 10.1186/1471-2229-14-99. View

Adrian M, Jeandet P . Effects of resveratrol on the ultrastructure of Botrytis cinerea conidia and biological significance in plant/pathogen interactions. Fitoterapia. 2012; 83(8):1345-50. DOI: 10.1016/j.fitote.2012.04.004. View

Alonso-Villaverde V, Voinesco F, Viret O, Spring J, Gindro K . The effectiveness of stilbenes in resistant Vitaceae: ultrastructural and biochemical events during Plasmopara viticola infection process. Plant Physiol Biochem. 2011; 49(3):265-74. DOI: 10.1016/j.plaphy.2010.12.010. View

Jeandet P, Clement C, Cordelier S . Regulation of resveratrol biosynthesis in grapevine: new approaches for disease resistance?. J Exp Bot. 2019; 70(2):375-378. PMC: 6322570. DOI: 10.1093/jxb/ery446. View

Divilov K, Barba P, Cadle-Davidson L, Reisch B . Single and multiple phenotype QTL analyses of downy mildew resistance in interspecific grapevines. Theor Appl Genet. 2018; 131(5):1133-1143. PMC: 5895686. DOI: 10.1007/s00122-018-3065-y. View

Viana A, Riaz S, Walker M . Genetic dissection of agronomic traits within a segregating population of breeding table grapes. Genet Mol Res. 2013; 12(2):951-64. DOI: 10.4238/2013.April.2.11. View

Vannozzi A, Dry I, Fasoli M, Zenoni S, Lucchin M . Genome-wide analysis of the grapevine stilbene synthase multigenic family: genomic organization and expression profiles upon biotic and abiotic stresses. BMC Plant Biol. 2012; 12:130. PMC: 3433347. DOI: 10.1186/1471-2229-12-130. View

Moroldo M, Paillard S, Marconi R, Fabrice L, Canaguier A, Cruaud C . A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance. BMC Plant Biol. 2008; 8:66. PMC: 2442077. DOI: 10.1186/1471-2229-8-66. View

10.

Jiang J, Xi H, Dai Z, Lecourieux F, Yuan L, Liu X . VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine. J Exp Bot. 2018; 70(2):715-729. PMC: 6322584. DOI: 10.1093/jxb/ery401. View

11.

Adam-Blondon A, Roux C, Claux D, Butterlin G, Merdinoglu D, This P . Mapping 245 SSR markers on the Vitis vinifera genome: a tool for grape genetics. Theor Appl Genet. 2004; 109(5):1017-27. DOI: 10.1007/s00122-004-1704-y. View

12.

Cui H, Tsuda K, Parker J . Effector-triggered immunity: from pathogen perception to robust defense. Annu Rev Plant Biol. 2014; 66:487-511. DOI: 10.1146/annurev-arplant-050213-040012. View

13.

Salmaso M, Malacarne G, Troggio M, Faes G, Stefanini M, Grando M . A grapevine (Vitis vinifera L.) genetic map integrating the position of 139 expressed genes. Theor Appl Genet. 2008; 116(8):1129-43. DOI: 10.1007/s00122-008-0741-3. View

14.

Marguerit E, Boury C, Manicki A, Donnart M, Butterlin G, Nemorin A . Genetic dissection of sex determinism, inflorescence morphology and downy mildew resistance in grapevine. Theor Appl Genet. 2009; 118(7):1261-78. DOI: 10.1007/s00122-009-0979-4. View

15.

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

16.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

17.

Venuti S, Copetti D, Foria S, Falginella L, Hoffmann S, Bellin D . Historical introgression of the downy mildew resistance gene Rpv12 from the Asian species Vitis amurensis into grapevine varieties. PLoS One. 2013; 8(4):e61228. PMC: 3625174. DOI: 10.1371/journal.pone.0061228. View

18.

Casagrande K, Falginella L, Castellarin S, Testolin R, Di Gaspero G . Defence responses in Rpv3-dependent resistance to grapevine downy mildew. Planta. 2011; 234(6):1097-109. DOI: 10.1007/s00425-011-1461-5. View

19.

Breuil A, Jeandet P, Adrian M, Chopin F, Pirio N, Meunier P . Characterization of a Pterostilbene Dehydrodimer Produced by Laccase of Botrytis cinerea. Phytopathology. 2008; 89(4):298-302. DOI: 10.1094/PHYTO.1999.89.4.298. View

20.

Huang Y, Bertrand Y, Guiraud J, Vialet S, Launay A, Cheynier V . Expression QTL mapping in grapevine--revisiting the genetic determinism of grape skin colour. Plant Sci. 2013; 207:18-24. DOI: 10.1016/j.plantsci.2013.02.011. View