PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2020 Jun 24

PMID 32574593

Citations 7

Authors

Jean-Francois Poulin

Milagros Pereira Luppi

Caitlyn Hofer

Giuliana Caronia

Pei-Ken Hsu

C Savio Chan

Rajeshwar Awatramani

Affiliations

Soon will be listed here.

Abstract

Lineage tracing aims to identify the progeny of a defined population of dividing progenitor cells, a daunting task in the developing central nervous system where thousands of cell types are generated. In mice, lineage analysis has been accomplished using Cre recombinase to indelibly label a defined progenitor population and its progeny. However, the interpretation of historical recombination events is hampered by the fact that driver genes are often expressed in both progenitors and postmitotic cells. Genetically inducible approaches provide temporal specificity but are afflicted by mosaicism and toxicity. Here, we present PRISM, a progenitor-restricted intersectional fate mapping approach in which Flp recombinase expression is both dependent on Cre and restricted to neural progenitors, thus circumventing the aforementioned confounds. This tool can be used in conjunction with existing Cre lines making it broadly applicable. We applied PRISM to resolve two developmentally important, but contentious, lineages-Shh and Cux2.

Citing Articles

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Application of Lineage Tracing in Central Nervous System Development and Regeneration.

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Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins.

Pereira Luppi M, Azcorra M, Caronia-Brown G, Poulin J, Gaertner Z, Gatica S Cell Rep. 2021; 37(6):109975.

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Dissociable Roles of Pallidal Neuron Subtypes in Regulating Motor Patterns.

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