Pax6 Organizes the Anterior Eye Segment by Guiding Two Distinct Neural Crest Waves

Overview

Journal PLoS Genet

Specialty Genetics

Date 2020 Jun 20

PMID 32555736

Citations 20

Authors

Masanari Takamiya

Johannes Stegmaier

Andrei Yu Kobitski

Benjamin Schott

Benjamin D Weger

Dimitra Margariti

Angel R Cereceda Delgado

Victor Gourain

Tim Scherr

Lixin Yang

Sebastian Sorge

Jens C Otte

Volker Hartmann

Jos van Wezel

Rainer Stotzka

Thomas Reinhard

Gunther Schlunck

Thomas Dickmeis

Sepand Rastegar

Ralf Mikut

Gerd Ulrich Nienhaus

Uwe Strahle

Affiliations

Soon will be listed here.

Abstract

Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGFβ signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment.

Citing Articles

Genetic analysis of medaka fish illuminates conserved and divergent roles of Pax6 in vertebrate eye development.

Mikula Mrstakova S, Kozmik Z Front Cell Dev Biol. 2024; 12:1448773.

PMID: 39512904 PMC: 11541176. DOI: 10.3389/fcell.2024.1448773.

Genetic and Developmental Divergence in the Neural Crest Program between Cichlid Fish Species.

Marconi A, Vernaz G, Karunaratna A, Ngochera M, Durbin R, Santos M Mol Biol Evol. 2024; 41(11).

PMID: 39412298 PMC: 11558072. DOI: 10.1093/molbev/msae217.

Keratin 8/18a.1 Expression Influences Embryonic Neural Crest Cell Dynamics and Contributes to Postnatal Corneal Regeneration in Zebrafish.

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Clinical and Genetic Correlation in Neurocristopathies: Bridging a Precision Medicine Gap.

Chatzi D, Kyriakoudi S, Dermitzakis I, Manthou M, Meditskou S, Theotokis P J Clin Med. 2024; 13(8).

PMID: 38673496 PMC: 11050951. DOI: 10.3390/jcm13082223.

Genetic and developmental divergence in the neural crest programme between cichlid fish species.

Marconi A, Vernaz G, Karunaratna A, Ngochera M, Durbin R, Santos M bioRxiv. 2024; .

PMID: 38352436 PMC: 10862805. DOI: 10.1101/2024.01.30.578004.

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