Molecular Characterisation of ILRUN, a Novel Inhibitor of Proinflammatory and Antimicrobial Cytokines

Overview

Journal Heliyon

Specialty Social Sciences

Date 2020 Jun 11

PMID 32518853

Citations 12

Authors

Rebecca L Ambrose

Aaron M Brice

Alessandro T Caputo

Marina R Alexander

Leon Tribolet

Yu Chih Liu

Timothy E Adams

Andrew G D Bean

Cameron R Stewart

Affiliations

Soon will be listed here.

Abstract

Regulation of type-I interferon (IFN) production is essential to the balance between antimicrobial defence and autoimmune disorders. The human protein-coding gene ILRUN (inflammation and lipid regulator with UBA-like and NBR1-like domains, previously C6orf106) was recently characterised as an inhibitor of antiviral and proinflammatory cytokine (interferon-alpha/beta and tumor necrosis factor alpha) transcription. Currently there is a paucity of information about the molecular characteristics of ILRUN, despite it being associated with several diseases including virus infection, coronary artery disease, obesity and cancer. Here, we characterise ILRUN as a highly phylogenetically conserved protein containing UBA-like and a NBR1-like domains that are both essential for inhibition of type-I interferon and tumor necrosis factor alpha) transcription in human cells. We also solved the crystal structure of the NBR1-like domain, providing insights into its potential role in ILRUN function. This study provides critical information for future investigations into the role of ILRUN in health and disease.

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