A Review on Microdevices for Isolating Circulating Tumor Cells

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2020 May 28

PMID 32456042

Citations 12

Authors

Kin Fong Lei

Affiliations

Soon will be listed here.

Abstract

Cancer metastasis is the primary cause of high mortality of cancer patients. Enumeration of circulating tumor cells (CTCs) in the bloodstream is a very important indicator to estimate the therapeutic outcome in various metastatic cancers. The aim of this article is to review recent developments on the CTC isolation technologies in microdevices. Based on the categories of biochemical and biophysical isolation approaches, a literature review and in-depth discussion will be included to provide an overview of this challenging topic. The current excellent developments suggest promising CTC isolation methods in order to establish a precise indicator of the therapeutic outcome of cancer patients.

Citing Articles

Microfluidic Technologies in Advancing Cancer Research.

Ajikumar A, Lei K Micromachines (Basel). 2025; 15(12.

PMID: 39770196 PMC: 11677295. DOI: 10.3390/mi15121444.

Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review.

Qiao Z, Teng X, Liu A, Yang W Micromachines (Basel). 2024; 15(6).

PMID: 38930676 PMC: 11206030. DOI: 10.3390/mi15060706.

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices.

Jou H, Ho H, Huang K, Chen C, Chen S, Lo P Int J Mol Sci. 2022; 23(23).

PMID: 36499466 PMC: 9736518. DOI: 10.3390/ijms232315139.

Numerical Study of a Centrifugal Platform for the Inertial Separation of Circulating Tumor Cells Using Contraction-Expansion Array Microchannels.

Ebrahimi S, Tahmasebipour M Arch Razi Inst. 2022; 77(2):647-660.

PMID: 36284940 PMC: 9548281. DOI: 10.22092/ARI.2022.357477.2046.

Skin Cancer Research Goes Digital: Looking for Biomarkers within the Droplets.

Dobre E, Constantin C, Neagu M J Pers Med. 2022; 12(7).

PMID: 35887633 PMC: 9323323. DOI: 10.3390/jpm12071136.

References

Wei R, Sun D, Yang H, Yan J, Zhang X, Zheng X . CTC clusters induced by heparanase enhance breast cancer metastasis. Acta Pharmacol Sin. 2018; 39(8):1326-1337. PMC: 6289387. DOI: 10.1038/aps.2017.189. View

Chiu T, Lei K, Hsieh C, Hsiao H, Wang H, Wu M . Development of a microfluidic-based optical sensing device for label-free detection of circulating tumor cells (CTCs) through their lactic acid metabolism. Sensors (Basel). 2015; 15(3):6789-806. PMC: 4435186. DOI: 10.3390/s150306789. View

Marrinucci D, Bethel K, Bruce R, Curry D, Hsieh B, Humphrey M . Case study of the morphologic variation of circulating tumor cells. Hum Pathol. 2006; 38(3):514-9. DOI: 10.1016/j.humpath.2006.08.027. View

Cohen S, Punt C, Iannotti N, Saidman B, Sabbath K, Gabrail N . Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer. J Clin Oncol. 2008; 26(19):3213-21. DOI: 10.1200/JCO.2007.15.8923. View

Yu M, Bardia A, Wittner B, Stott S, Smas M, Ting D . Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science. 2013; 339(6119):580-4. PMC: 3760262. DOI: 10.1126/science.1228522. View

Sekine J, Luo S, Wang S, Zhu B, Tseng H, Yu H . Functionalized conducting polymer nanodots for enhanced cell capturing: the synergistic effect of capture agents and nanostructures. Adv Mater. 2011; 23(41):4788-92. DOI: 10.1002/adma.201102151. View

Cristofanilli M, Budd G, Ellis M, Stopeck A, Matera J, Miller M . Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med. 2004; 351(8):781-91. DOI: 10.1056/NEJMoa040766. View

de Bono J, Scher H, Montgomery R, Parker C, Miller M, Tissing H . Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res. 2008; 14(19):6302-9. DOI: 10.1158/1078-0432.CCR-08-0872. View

Plaks V, Koopman C, Werb Z . Cancer. Circulating tumor cells. Science. 2013; 341(6151):1186-8. PMC: 3842225. DOI: 10.1126/science.1235226. View

10.

Sarioglu A, Aceto N, Kojic N, Donaldson M, Zeinali M, Hamza B . A microfluidic device for label-free, physical capture of circulating tumor cell clusters. Nat Methods. 2015; 12(7):685-91. PMC: 4490017. DOI: 10.1038/nmeth.3404. View

11.

Tanaka T, Ishikawa T, Numayama-Tsuruta K, Imai Y, Ueno H, Matsuki N . Separation of cancer cells from a red blood cell suspension using inertial force. Lab Chip. 2012; 12(21):4336-43. DOI: 10.1039/c2lc40354d. View

12.

Bidard F, Proudhon C, Pierga J . Circulating tumor cells in breast cancer. Mol Oncol. 2016; 10(3):418-30. PMC: 5528978. DOI: 10.1016/j.molonc.2016.01.001. View

13.

Earhart C, Hughes C, Gaster R, Ooi C, Wilson R, Zhou L . Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochips. Lab Chip. 2013; 14(1):78-88. PMC: 4144998. DOI: 10.1039/c3lc50580d. View

14.

Talasaz A, Powell A, Huber D, Berbee J, Roh K, Yu W . Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device. Proc Natl Acad Sci U S A. 2009; 106(10):3970-5. PMC: 2645911. DOI: 10.1073/pnas.0813188106. View

15.

Tan S, Yobas L, Lee G, Ong C, Lim C . Microdevice for the isolation and enumeration of cancer cells from blood. Biomed Microdevices. 2009; 11(4):883-92. DOI: 10.1007/s10544-009-9305-9. View

16.

Moon H, Kwon K, Kim S, Han H, Sohn J, Lee S . Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP). Lab Chip. 2011; 11(6):1118-25. DOI: 10.1039/c0lc00345j. View

17.

Coumans F, van Dalum G, Beck M, Terstappen L . Filter characteristics influencing circulating tumor cell enrichment from whole blood. PLoS One. 2013; 8(4):e61770. PMC: 3634026. DOI: 10.1371/journal.pone.0061770. View

18.

Zheng S, Lin H, Liu J, Balic M, Datar R, Cote R . Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. J Chromatogr A. 2007; 1162(2):154-61. DOI: 10.1016/j.chroma.2007.05.064. View

19.

Pethig R . Review article-dielectrophoresis: status of the theory, technology, and applications. Biomicrofluidics. 2010; 4(2). PMC: 2917862. DOI: 10.1063/1.3456626. View

20.

Chen S, El-Heliebi A, Tauber G, Langsenlehner T, Potscher M, Kashofer K . Catch and Release: rare cell analysis from a functionalised medical wire. Sci Rep. 2017; 7:43424. PMC: 5324062. DOI: 10.1038/srep43424. View