Numerical Study of a Centrifugal Platform for the Inertial Separation of Circulating Tumor Cells Using Contraction-Expansion Array Microchannels

Overview

Journal Arch Razi Inst

Specialties Allergy & Immunology
General Medicine
Veterinary Medicine

Date 2022 Oct 26

PMID 36284940

Authors

Sh Ebrahimi

M Tahmasebipour

Affiliations

Soon will be listed here.

Abstract

Label-free inertial separation of the circulating tumor cells (CTCs) has attracted significant attention recently. The present study proposed a centrifugal platform for the inertial separation of the CTCs from the white blood cells. Particle trajectories of the contraction-expansion array (CEA) microchannels were analyzed by the finite element method. Four expansion geometries (i.e., circular, rectangular, trapezoidal, and triangular) were compared to explore their differences in separation possibilities. Different operational and geometrical parameters were investigated to achieve maximum separation efficiency. Results indicated that the trapezoidal CEA microchannel with ten expansions and a 100 µm channel depth had the best separation performance at an angular velocity of 100 rad/s. Reynolds number of 47 was set as the optimum value to apply minimum shear stress on the CTCs leading to 100% efficiency and 95% purity. Furthermore, the proposed system was simulated for whole blood by considering the red blood cells.

Citing Articles

Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review.

Farahinia A, Zhang W, Badea I Sensors (Basel). 2023; 23(11).

PMID: 37300027 PMC: 10256097. DOI: 10.3390/s23115300.

References

Herath S, Razavi Bazaz S, Monkman J, Warkiani M, Richard D, OByrne K . Circulating tumor cell clusters: Insights into tumour dissemination and metastasis. Expert Rev Mol Diagn. 2020; 20(11):1139-1147. DOI: 10.1080/14737159.2020.1846523. View

Madadelahi M, Acosta-Soto L, Hosseini S, Martinez-Chapa S, Madou M . Mathematical modeling and computational analysis of centrifugal microfluidic platforms: a review. Lab Chip. 2020; 20(8):1318-1357. DOI: 10.1039/c9lc00775j. View

Nasiri R, Shamloo A, Akbari J, Tebon P, Dokmeci M, Ahadian S . Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis. Micromachines (Basel). 2020; 11(7). PMC: 7407509. DOI: 10.3390/mi11070699. View

Lei K . A Review on Microdevices for Isolating Circulating Tumor Cells. Micromachines (Basel). 2020; 11(5). PMC: 7281722. DOI: 10.3390/mi11050531. View

Guan G, Wu L, Bhagat A, Li Z, Chen P, Chao S . Spiral microchannel with rectangular and trapezoidal cross-sections for size based particle separation. Sci Rep. 2013; 3:1475. PMC: 3600595. DOI: 10.1038/srep01475. View

Che J, Yu V, Dhar M, Renier C, Matsumoto M, Heirich K . Classification of large circulating tumor cells isolated with ultra-high throughput microfluidic Vortex technology. Oncotarget. 2016; 7(11):12748-60. PMC: 4914319. DOI: 10.18632/oncotarget.7220. View

Hao S, Wan Y, Xia Y, Zou X, Zheng S . Size-based separation methods of circulating tumor cells. Adv Drug Deliv Rev. 2018; 125:3-20. DOI: 10.1016/j.addr.2018.01.002. View

Ying Y, Lin Y . Inertial Focusing and Separation of Particles in Similar Curved Channels. Sci Rep. 2019; 9(1):16575. PMC: 6851121. DOI: 10.1038/s41598-019-52983-z. View

Mirzaaghaian A, Ramiar A, Ranjbar A, Warkiani M . Application of level-set method in simulation of normal and cancer cells deformability within a microfluidic device. J Biomech. 2020; 112:110066. DOI: 10.1016/j.jbiomech.2020.110066. View

10.

Lee A, Park J, Lim M, Sunkara V, Kim S, Kim G . All-in-one centrifugal microfluidic device for size-selective circulating tumor cell isolation with high purity. Anal Chem. 2014; 86(22):11349-56. DOI: 10.1021/ac5035049. View

11.

Lee M, Shin J, Bae C, Choi S, Park J . Label-free cancer cell separation from human whole blood using inertial microfluidics at low shear stress. Anal Chem. 2013; 85(13):6213-8. DOI: 10.1021/ac4006149. View

12.

Zhou J, Kulasinghe A, Bogseth A, OByrne K, Punyadeera C, Papautsky I . Isolation of circulating tumor cells in non-small-cell-lung-cancer patients using a multi-flow microfluidic channel. Microsyst Nanoeng. 2019; 5:8. PMC: 6387977. DOI: 10.1038/s41378-019-0045-6. View

13.

Park J, Kim M, Moon H, Yoo C, Park D, Kim Y . Fully automated circulating tumor cell isolation platform with large-volume capacity based on lab-on-a-disc. Anal Chem. 2014; 86(8):3735-42. DOI: 10.1021/ac403456t. View

14.

Dhar M, Pao E, Renier C, Go D, Che J, Montoya R . Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells. Sci Rep. 2016; 6:35474. PMC: 5064381. DOI: 10.1038/srep35474. View

15.

Di Carlo D, Irimia D, Tompkins R, Toner M . Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci U S A. 2007; 104(48):18892-7. PMC: 2141878. DOI: 10.1073/pnas.0704958104. View

16.

Warkiani M, Khoo B, Wu L, Tay A, Bhagat A, Han J . Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics. Nat Protoc. 2015; 11(1):134-48. DOI: 10.1038/nprot.2016.003. View

17.

Al-Halhouli A, Doofesh Z, Albagdady A, Dietzel A . High-Efficiency Small Sample Microparticle Fractionation on a Femtosecond Laser-Machined Microfluidic Disc. Micromachines (Basel). 2020; 11(2). PMC: 7074639. DOI: 10.3390/mi11020151. View

18.

Dalili A, Samiei E, Hoorfar M . A review of sorting, separation and isolation of cells and microbeads for biomedical applications: microfluidic approaches. Analyst. 2018; 144(1):87-113. DOI: 10.1039/c8an01061g. View