The Rho-associated Kinase Inhibitor Fasudil Can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 May 13

PMID 32396545

Citations 13

Authors

Seongjun So

Yeonmi Lee

Jiwan Choi

Seoon Kang

Ji-Yoon Lee

Julie Hwang

Joosung Shin

James R Dutton

Eul-Ju Seo

Beom Hee Lee

Chong Jai Kim

Shoukhrat Mitalipov

Soo Jin Oh

Eunju Kang

Affiliations

Soon will be listed here.

Abstract

Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.

Citing Articles

Comparison of Natural Killer Cells Differentiated from Various Pluripotent Stem Cells.

Han J, Son H, Jung D, Kim K, Jin C, Hwang H Int J Mol Sci. 2024; 25(15).

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Post-thaw application of ROCK-inhibitors increases cryopreserved T-cell yield.

Gonzalez-Martinez N, Gibson M RSC Med Chem. 2023; 14(10):2058-2067.

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Therapeutic Potency of Induced Pluripotent Stem-Cell-Derived Corneal Endothelial-like Cells for Corneal Endothelial Dysfunction.

So S, Park Y, Kang S, Han J, Sunwoo J, Lee W Int J Mol Sci. 2023; 24(1).

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Mitochondrial genome mutations and neuronal dysfunction of induced pluripotent stem cells derived from patients with Alzheimer's disease.

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