An Updated Protocol for the Cost-effective and Weekend-free Culture of Human Induced Pluripotent Stem Cells

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 Mar 31

PMID 33786455

Citations 8

Authors

Davi Marco Lyra-Leite

Hananeh Fonoudi

Mennat Gharib

Paul W Burridge

Affiliations

Soon will be listed here.

Abstract

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).

Citing Articles

Examining patient-specific responses to PARP inhibitors in a novel, human induced pluripotent stem cell-based model of breast cancer.

Weddle C, Blancard M, Uche N, Pongpamorn P, Cejas R, Burridge P NPJ Precis Oncol. 2025; 9(1):53.

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Disruption of TGF-β signaling pathway is required to mediate effective killing of hepatocellular carcinoma by human iPSC-derived NK cells.

Lakshmi Thangaraj J, Coffey M, Lopez E, Kaufman D Cell Stem Cell. 2024; 31(9):1327-1343.e5.

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Systematic optimization of culture media for maintenance of human induced pluripotent stem cells using the response surface methodology.

Mousavi Mirkalaei S, Farivar S Heliyon. 2024; 10(12):e32558.

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Heparan Sulfate Chain-Conjugated Laminin-E8 Fragments Advance Paraxial Mesodermal Differentiation Followed by High Myogenic Induction from hiPSCs.

Zhao M, Taniguchi Y, Shimono C, Jonouchi T, Cheng Y, Shimizu Y Adv Sci (Weinh). 2024; 11(26):e2308306.

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Independent compartmentalization of functional, metabolic, and transcriptional maturation of hiPSC-derived cardiomyocytes.

Fetterman K, Blancard M, Lyra-Leite D, Vanoye C, Fonoudi H, Jouni M Cell Rep. 2024; 43(5):114160.

PMID: 38678564 PMC: 11247623. DOI: 10.1016/j.celrep.2024.114160.

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