Prevalence and Molecular Characterization of Species in Poultry in Bangladesh

Overview

Journal One Health

Specialty Infectious Diseases

Date 2020 May 6

PMID 32368610

Citations 13

Authors

Mohammad Hazzaz Bin Kabir

Yongmei Han

Seung-Hun Lee

Arifin Budiman Nugraha

Frances Recuenco

Fumi Murakoshi

Xuenan Xuan

Kentaro Kato

Affiliations

Soon will be listed here.

Abstract

is an opportunistic parasite that has been reported in >30 avian hosts worldwide, however, there is no information regarding spp. in poultry in Bangladesh. Accordingly, we investigated the prevalence of spp. in poultry at open live bird markets in Bangladesh. A total of 197 samples were randomly collected from poultry at open live bird markets in Bangladesh and screened for the detection of Initial microscopic examination revealed spp. was observed in 19.8% (39/197) of the poultry specimens. Subsequent nested PCR targeting the 18S rRNA gene revealed that 15.7% (31/197) of the samples were positive. Of these 31 samples, 17 were (8.7%), 12 were (6.0%), and 2 were (1.0%). Nucleotide sequence analysis of the GP60 gene of the revealed that two subtypes (IIIbA21G1R1 and IIIbA23G1R1), which were found in broiler, native and sonali chickens and a pigeon, matched those previously reported in humans and poultry. We identified two novel subtypes (IIIbA21G2R1 and IIIbA20G2R1) in sonali chickens, a broiler chicken and a layer chicken. We also amplified the GP60 gene of and found two subtypes (IIaA11G2R1 and IIaA13G2R1) in a sonali and a broiler chicken that were previously reported in calf. These findings suggest that poultry can be a source of cryptosporidial infections for humans and animals in Bangladesh. This is the first molecular investigation of genotypes and subtypes in poultry at open live bird markets in Bangladesh.

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