Mechanism of T-cell Help in the Immune Response to Soluble Protein Antigens II. Reconstitution of Primary and Secondary in Vitro Immune Responses to Dinitrophenyl-carrier Conjugates by T-cell-replacing Factor

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1977 May 1

PMID 323405

Citations 8

Authors

T H Hunig

A Schimpl

E WECKER

Affiliations

Soon will be listed here.

Abstract

Spleen cells of dinitrophenyl keyhole limpet hemocyanin (DNPKLH) primed and boosted mice produced a nonantigen-specific helper factor upon in vitro challenge with DNPKLH. This helper factor displays all of the biological characteristics so far described for TRF produced by allogeneic or Concanavalin A stimulation of mouse spleen cells. It restores the primary anti-SRBC response in nude spleen cultures following the same kinetics of action as T-cell-replacing factor (TRF). Conversely, TRF restores the primary in vitro immune response of nude spleen cultures to DNPKLH. TRF also restores the secondary anti-hapten IgG response of T-cell-deprived spleen cell cultures derived from DNPKLH primed and boosted mice. Here the need for carrier specificity is fully overcome. The data therefore suggest that TRF, as a nonantigen-specific maturation signal, is involved in the primary and secondary immune responses to both particulate and soluble antigens.

Citing Articles

Helper activity of T lymphocytes which have been stimulated by keyhole limpet haemocyanin in vitro.

Ward K Immunology. 1981; 43(1):111-6.

PMID: 6972901 PMC: 1555193.

Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion.

Parker D, Wadsworth D, Schneider G J Exp Med. 1980; 152(1):138-50.

PMID: 6967509 PMC: 2185896. DOI: 10.1084/jem.152.1.138.

The role of H-2-linked genes in helper T cell function. VII. Expression of I region and immune response genes by B cells in bystander help assays.

Marrack P, Kappler J J Exp Med. 1980; 152(5):1274-88.

PMID: 6159447 PMC: 2185988. DOI: 10.1084/jem.152.5.1274.

Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.

Mita S, Harada N, Naomi S, Hitoshi Y, Sakamoto K, Akagi M J Exp Med. 1988; 168(3):863-78.

PMID: 3262707 PMC: 2189030. DOI: 10.1084/jem.168.3.863.

In vitro analysis of allogeneic lymphocyte interaction. II. I-region control of the activity of a B-cell-derived H-2-restricted allogeneic effect factor and its receptor during B-cell activation.

Delovitch T, Biggin J, Fung F J Exp Med. 1978; 147(4):1198-212.

PMID: 418135 PMC: 2184251. DOI: 10.1084/jem.147.4.1198.

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