Dynamic Interplay of Host and Pathogens in an Avian Whole-Blood Model

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2020 Apr 17

PMID 32296424

Citations 7

Authors

Sravya Sreekantapuram

Teresa Lehnert

Maria T E Prausse

Angela Berndt

Christian Berens

Marc Thilo Figge

Ilse D Jacobsen

Affiliations

Soon will be listed here.

Abstract

Microbial survival in blood is an essential step toward the development of disseminated diseases and blood stream infections. For poultry, however, little is known about the interactions of host cells and pathogens in blood. We established an chicken whole-blood infection assay as a tool to analyze interactions between host cells and three model pathogens, , and . Following a systems biology approach, we complemented the experimental measurements with functional and quantitative immune characteristics by virtual infection modeling. All three pathogens were killed in whole blood, but each to a different extent and with different kinetics. Monocytes, and to a lesser extent heterophils, associated with pathogens. Both association with host cells and transcriptional activation of genes encoding immune-associated functions differed depending on both the pathogen and the genetic background of the chickens. Our results provide first insights into quantitative interactions of three model pathogens with different immune cell populations in avian blood, demonstrating a broad spectrum of different characteristics during the immune response that depends on the pathogen and the chicken line.

Citing Articles

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Interaction of Salmonella Gallinarum and Salmonella Enteritidis with peripheral leucocytes of hens with different laying performance.

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Comparative assessment of immune evasion mechanisms in human whole-blood infection assays by a systems biology approach.

Lehnert T, Prausse M, Hunniger K, Praetorius J, Kurzai O, Figge M PLoS One. 2021; 16(4):e0249372.

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