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Assembly and Analysis of the Complete Mitochondrial Genome of

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Journal Plants (Basel)
Date 2020 Apr 12
PMID 32276324
Citations 10
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Abstract

Shepherd's purse () is a cosmopolitan annual weed and a promising model plant for studying allopolyploidization in the evolution of angiosperms. Though plant mitochondrial genomes are a valuable source of genetic information, they are hard to assemble. At present, only the complete mitogenome of is available out of all species of the genus Capsella. In this work, we have assembled the complete mitogenome of using high-precision PacBio SMRT third-generation sequencing technology. It is 287,799 bp long and contains 32 protein-coding genes, 3 rRNAs, 25 tRNAs corresponding to 15 amino acids, and 8 open reading frames (ORFs) supported by RNAseq data. Though many repeat regions have been found, none of them is longer than 1 kbp, and the most frequent structural variant originated from these repeats is present in only 4% of the mitogenome copies. The mitochondrial DNA sequence of differs from , but not from , by two long inversions, suggesting that could be its maternal progenitor species. In total, 377 C to U RNA editing sites have been detected. All genes except and contain RNA editing sites, and most of them lead to non-synonymous changes of amino acids. Most of the identified RNA editing sites are identical to corresponding RNA editing sites in .

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