Complete Sequence of Kenaf (Hibiscus Cannabinus) Mitochondrial Genome and Comparative Analysis with the Mitochondrial Genomes of Other Plants

Overview

Journal Sci Rep

Specialty Science

Date 2018 Aug 26

PMID 30143661

Citations 30

Authors

Xiaofang Liao

Yanhong Zhao

Xiangjun Kong

Aziz Khan

Bujin Zhou

Dongmei Liu

Muhammad Haneef Kashif

Peng Chen

Hong Wang

Ruiyang Zhou

Affiliations

Soon will be listed here.

Abstract

Plant mitochondrial (mt) genomes are species specific due to the vast of foreign DNA migration and frequent recombination of repeated sequences. Sequencing of the mt genome of kenaf (Hibiscus cannabinus) is essential for elucidating its evolutionary characteristics. In the present study, single-molecule real-time sequencing technology (SMRT) was used to sequence the complete mt genome of kenaf. Results showed that the complete kenaf mt genome was 569,915 bp long and consisted of 62 genes, including 36 protein-coding, 3 rRNA and 23 tRNA genes. Twenty-five introns were found among nine of the 36 protein-coding genes, and five introns were trans-spliced. A comparative analysis with other plant mt genomes showed that four syntenic gene clusters were conserved in all plant mtDNAs. Fifteen chloroplast-derived fragments were strongly associated with mt genes, including the intact sequences of the chloroplast genes psaA, ndhB and rps7. According to the plant mt genome evolution analysis, some ribosomal protein genes and succinate dehydrogenase genes were frequently lost during the evolution of angiosperms. Our data suggest that the kenaf mt genome retained evolutionarily conserved characteristics. Overall, the complete sequencing of the kenaf mt genome provides additional information and enhances our better understanding of mt genomic evolution across angiosperms.

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