Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Overview

Journal Front Bioeng Biotechnol

Date 2020 Apr 9

PMID 32266240

Citations 41

Authors

Nadanai Laohakunakorn

Laura Grasemann

Barbora Lavickova

Gregoire Michielin

Amir Shahein

Zoe Swank

Sebastian J Maerkl

Affiliations

Soon will be listed here.

Abstract

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner.

Citing Articles

Challenges in observing transcription-translation for bottom-up synthetic biology.

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Roset Julia L, Grasemann L, Stellacci F, Maerkl S ACS Synth Biol. 2025; 14(1):290-295.

PMID: 39757539 PMC: 11744927. DOI: 10.1021/acssynbio.4c00618.

ATP Regeneration from Pyruvate in the PURE System.

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Cell-Free Gene Expression: Methods and Applications.

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Regulatory Components for Bacterial Cell-Free Systems Engineering.

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