Cell-free Expression of RuBisCO for ATP Production in the Synthetic Cells

Overview

Journal Synth Biol (Oxf)

Publisher Oxford University Press

Date 2023 Dec 27

PMID 38149045

Authors

Shugo Sugii

Katsumi Hagino

Ryo Mizuuchi

Norikazu Ichihashi

Affiliations

Soon will be listed here.

Abstract

Recent advances in bottom-up synthetic biology have made it possible to reconstitute cellular systems from non-living components, yielding artificial cells with potential applications in industry, medicine and basic research. Although a variety of cellular functions and components have been reconstituted in previous studies, sustained biological energy production remains a challenge. ATP synthesis via ribulose-1,5-diphosphate carboxylase/oxygenase (RuBisCO), a central enzyme in biological CO fixation, holds potential as an energy production system, but its feasibility in a cell-free expression system has not yet been tested. In this study, we test RuBisCO expression and its activity-mediated ATP synthesis in a reconstituted -based cell-free translation system. We then construct a system in which ATP is synthesized by RuBisCO activity in giant vesicles and used as energy for translation reactions. These results represent an advance toward independent energy production in artificial cells. .

Citing Articles

Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.

Sato G, Miyazawa S, Doi N, Fujiwara K Molecules. 2024; 29(13).

PMID: 38998908 PMC: 11243612. DOI: 10.3390/molecules29132956.

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