Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2020 Mar 12

PMID 32160149

Citations 357

Authors

Jennifer Harcourt

Azaibi Tamin

Xiaoyan Lu

Shifaq Kamili

Senthil K Sakthivel

Janna Murray

Krista Queen

Ying Tao

Clinton R Paden

Jing Zhang

Yan Li

Anna Uehara

Haibin Wang

Cynthia Goldsmith

Hannah A Bullock

Lijuan Wang

Brett Whitaker

Brian Lynch

Rashi Gautam

Craig Schindewolf

Kumari G Lokugamage

Dionna Scharton

Jessica A Plante

Divya Mirchandani

Steven G Widen

Krishna Narayanan

Shinji Makino

Thomas G Ksiazek

Kenneth S Plante

Scott C Weaver

Stephen Lindstrom

Suxiang Tong

Vineet D Menachery

Natalie J Thornburg

Affiliations

Soon will be listed here.

Abstract

The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.

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