Imaging of Corneal Nerves and Cellular Structures in Mice with Gabor-domain Optical Coherence Microscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2020 Mar 6

PMID 32133220

Citations 9

Authors

Cristina Canavesi

Andrea Cogliati

Amanda Mietus

Yue Qi

Jesse Schallek

Jannick P Rolland

Holly B Hindman

Affiliations

Soon will be listed here.

Abstract

Gabor-domain optical coherence microscopy (GDOCM) demonstrated corneal imaging with cellular resolution and differentiation in mice over a field of view of 1 mm. Contact and non-contact imaging was conducted on six healthy and six hyperglycemic C57BL/6J mice. Cellular resolution in the 3D GDOCM images was achieved after motion correction. Corneal nerve fibers were traced and their lengths and branches calculated. Noncontact, label-free imaging of corneal nerves has clinical utility in health and disease, and in transplant evaluation. To the authors' knowledge, this is the first report of 3D corneal imaging in mice with the capability to resolve nerve fibers using a non-contact imaging modality.

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