250 KHz, 1.5 µm Resolution SD-OCT for Cellular Imaging of the Human Cornea

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2019 May 9

PMID 31065450

Citations 41

Authors

Bingyao Tan

Zohreh Hosseinaee

Le Han

Olivera Kralj

Luigina Sorbara

Kostadinka Bizheva

Affiliations

Soon will be listed here.

Abstract

We present the first spectral domain optical coherence tomography (SD-OCT) system that combines an isotropic imaging resolution of ~1.5 µm in biological tissue with a 250 kHz image acquisition rate, for non-contact, volumetric imaging of the cellular structure of the human cornea. OCT images of the healthy human cornea acquired with this system reveal the cellular structure of the corneal epithelium, cellular debris and mucin clusters in the tear film, the shape, size and spatial distribution of the sub-basal corneal nerves and keratocytes in the corneal stroma, as well as reflections from endothelial nuclei. The corneal images presented here demonstrate the potential clinical value of the new high speed, high resolution OCT system for non-invasive diagnostics and monitoring the treatment of corneal diseases.

Citing Articles

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References

Whitcher J, Srinivasan M, Upadhyay M . Corneal blindness: a global perspective. Bull World Health Organ. 2001; 79(3):214-21. PMC: 2566379. View

Jalbert I, Stapleton F, Papas E, Sweeney D, Coroneo M . In vivo confocal microscopy of the human cornea. Br J Ophthalmol. 2003; 87(2):225-36. PMC: 1771516. DOI: 10.1136/bjo.87.2.225. View

Dubois A, Grieve K, Moneron G, Lecaque R, Vabre L, Boccara C . Ultrahigh-resolution full-field optical coherence tomography. Appl Opt. 2004; 43(14):2874-83. DOI: 10.1364/ao.43.002874. View

Grieve K, Paques M, Dubois A, Sahel J, Boccara C, Le Gargasson J . Ocular tissue imaging using ultrahigh-resolution, full-field optical coherence tomography. Invest Ophthalmol Vis Sci. 2004; 45(11):4126-31. DOI: 10.1167/iovs.04-0584. View

Ding Z, Ren H, Zhao Y, Nelson J, Chen Z . High-resolution optical coherence tomography over a large depth range with an axicon lens. Opt Lett. 2007; 27(4):243-5. DOI: 10.1364/ol.27.000243. View

Povazay B, Bizheva K, Unterhuber A, Hermann B, Sattmann H, Fercher A . Submicrometer axial resolution optical coherence tomography. Opt Lett. 2007; 27(20):1800-2. DOI: 10.1364/ol.27.001800. View

Potsaid B, Gorczynska I, Srinivasan V, Chen Y, Jiang J, Cable A . Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second. Opt Express. 2008; 16(19):15149-69. PMC: 2743204. DOI: 10.1364/oe.16.015149. View

Ramos J, Li Y, Huang D . Clinical and research applications of anterior segment optical coherence tomography - a review. Clin Exp Ophthalmol. 2008; 37(1):81-9. PMC: 2706099. DOI: 10.1111/j.1442-9071.2008.01823.x. View

Schmoll T, Kolbitsch C, Leitgeb R . Ultra-high-speed volumetric tomography of human retinal blood flow. Opt Express. 2009; 17(5):4166-76. DOI: 10.1364/oe.17.004166. View

10.

Huang D, Swanson E, Lin C, Schuman J, Stinson W, Chang W . Optical coherence tomography. Science. 1991; 254(5035):1178-81. PMC: 4638169. DOI: 10.1126/science.1957169. View

11.

Niederer R, McGhee C . Clinical in vivo confocal microscopy of the human cornea in health and disease. Prog Retin Eye Res. 2009; 29(1):30-58. DOI: 10.1016/j.preteyeres.2009.11.001. View

12.

Burton M . Prevention, treatment and rehabilitation. Community Eye Health. 2010; 22(71):33-5. PMC: 2823104. View

13.

Hutchings N, Simpson T, Hyun C, Moayed A, Hariri S, Sorbara L . Swelling of the human cornea revealed by high-speed, ultrahigh-resolution optical coherence tomography. Invest Ophthalmol Vis Sci. 2010; 51(9):4579-84. DOI: 10.1167/iovs.09-4676. View

14.

Wieser W, Biedermann B, Klein T, Eigenwillig C, Huber R . Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second. Opt Express. 2010; 18(14):14685-704. DOI: 10.1364/OE.18.014685. View

15.

Tavakoli M, Malik R . Corneal confocal microscopy: a novel non-invasive technique to quantify small fibre pathology in peripheral neuropathies. J Vis Exp. 2011; (47). PMC: 3182640. DOI: 10.3791/2194. View

16.

Liu L, Gardecki J, Nadkarni S, Toussaint J, Yagi Y, Bouma B . Imaging the subcellular structure of human coronary atherosclerosis using micro-optical coherence tomography. Nat Med. 2011; 17(8):1010-4. PMC: 3151347. DOI: 10.1038/nm.2409. View

17.

Wang J, Shousha M, Perez V, Karp C, Yoo S, Shen M . Ultra-high resolution optical coherence tomography for imaging the anterior segment of the eye. Ophthalmic Surg Lasers Imaging. 2011; 42 Suppl:S15-27. DOI: 10.3928/15428877-20110627-02. View

18.

An L, Li P, Shen T, Wang R . High speed spectral domain optical coherence tomography for retinal imaging at 500,000 A‑lines per second. Biomed Opt Express. 2011; 2(10):2770-83. PMC: 3191444. DOI: 10.1364/BOE.2.002770. View

19.

Yadav R, Lee K, Rolland J, Zavislan J, Aquavella J, Yoon G . Micrometer axial resolution OCT for corneal imaging. Biomed Opt Express. 2011; 2(11):3037-46. PMC: 3207373. DOI: 10.1364/BOE.2.003037. View

20.

Karimi A, Wong A, Bizheva K . Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical coherence tomograms. Biomed Opt Express. 2011; 2(10):2905-16. PMC: 3191454. DOI: 10.1364/BOE.2.002905. View