Transcriptome and Metabolite Analyses Provide Insights into Zigzag-shaped Stem Formation in Tea Plants (Camellia Sinensis)

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2020 Mar 6

PMID 32131737

Citations 8

Authors

Hongli Cao

Feiquan Wang

Hongzheng Lin

Yijun Ye

Yucheng Zheng

Jiamin Li

Zhilong Hao

Naixing Ye

Chuan Yue

Affiliations

Soon will be listed here.

Abstract

Background: Shoot orientation is important for plant architecture formation, and zigzag-shaped shoots are a special trait found in many plants. Zigzag-shaped shoots have been selected and thoroughly studied in Arabidopsis; however, the regulatory mechanism underlying zigzag-shaped shoot development in other plants, especially woody plants, is largely unknown.

Results: In this study, tea plants with zigzag-shaped shoots, namely, Qiqu (QQ) and Lianyuanqiqu (LYQQ), were investigated and compared with the erect-shoot tea plant Meizhan (MZ) in an attempt to reveal the regulation of zigzag-shaped shoot formation. Tissue section observation showed that the cell arrangement and shape of zigzag-shaped stems were aberrant compared with those of normal shoots. Moreover, a total of 2175 differentially expressed genes (DEGs) were identified from the zigzag-shaped shoots of the tea plants QQ and LYQQ compared to the shoots of MZ using transcriptome sequencing, and the DEGs involved in the "Plant-pathogen interaction", "Phenylpropanoid biosynthesis", "Flavonoid biosynthesis" and "Linoleic acid metabolism" pathways were significantly enriched. Additionally, the DEGs associated with cell expansion, vesicular trafficking, phytohormones, and transcription factors were identified and analysed. Metabolomic analysis showed that 13 metabolites overlapped and were significantly changed in the shoots of QQ and LYQQ compared to MZ.

Conclusions: Our results suggest that zigzag-shaped shoot formation might be associated with the gravitropism response and polar auxin transport in tea plants. This study provides a valuable foundation for further understanding the regulation of plant architecture formation and for the cultivation and application of horticultural plants in the future.

Citing Articles

High-density genetic map construction and QTL mapping of a zigzag-shaped stem trait in tea plant (Camellia sinensis).

Liu D, Ye Y, Tang R, Gong Y, Chen S, Zhang C BMC Plant Biol. 2024; 24(1):382.

PMID: 38724900 PMC: 11080114. DOI: 10.1186/s12870-024-05082-9.

Bulked Segregant RNA-Seq Reveals Different Gene Expression Patterns and Mutant Genes Associated with the Zigzag Pattern of Tea Plants ().

Ye Y, Liu D, Tang R, Gong Y, Zhang C, Mei P Int J Mol Sci. 2024; 25(8).

PMID: 38674133 PMC: 11049935. DOI: 10.3390/ijms25084549.

Genome-Wide Analysis of the Gene Family and Their Expression Pattern Analysis in Tea Plant ().

Shang X, Han Z, Zhang D, Wang Y, Qin H, Zou Z Front Plant Sci. 2022; 13:840350.

PMID: 35845692 PMC: 9284231. DOI: 10.3389/fpls.2022.840350.

Genome-Wide Identification and Characterization of Transcription Factor Gene Family Members Reveal Their Diverse Functions in Tea Plant ().

Yue C, Chen Q, Hu J, Li C, Luo L, Zeng L Front Plant Sci. 2022; 13:947072.

PMID: 35845671 PMC: 9280663. DOI: 10.3389/fpls.2022.947072.

The chromosome-level genome provides insight into the molecular mechanism underlying the tortuous-branch phenotype of Prunus mume.

Zheng T, Li P, Zhuo X, Liu W, Qiu L, Li L New Phytol. 2021; 235(1):141-156.

PMID: 34861048 PMC: 9299681. DOI: 10.1111/nph.17894.

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