High-density Genetic Map Construction and QTL Mapping of a Zigzag-shaped Stem Trait in Tea Plant (Camellia Sinensis)

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2024 May 9

PMID 38724900

Authors

Dingding Liu

Yuanyuan Ye

Rongjin Tang

Yang Gong

Si Chen

Chenyu Zhang

Piao Mei

Jiedan Chen

Liang Chen

Chunlei Ma

Affiliations

Soon will be listed here.

Abstract

The highly unique zigzag-shaped stem phenotype in tea plants boasts significant ornamental value and is exceptionally rare. To investigate the genetic mechanism behind this trait, we developed BC artificial hybrid populations. Our genetic analysis revealed the zigzag-shaped trait as a qualitative trait. Utilizing whole-genome resequencing, we constructed a high-density genetic map from the BC population, incorporating 5,250 SNP markers across 15 linkage groups, covering 3,328.51 cM with an average marker interval distance of 0.68 cM. A quantitative trait locus (QTL) for the zigzag-shaped trait was identified on chromosome 4, within a 61.2 to 97.2 Mb range, accounting for a phenotypic variation explained (PVE) value of 13.62%. Within this QTL, six candidate genes were pinpointed. To better understand their roles, we analyzed gene expression in various tissues and individuals with erect and zigzag-shaped stems. The results implicated CsXTH (CSS0035625) and CsCIPK14 (CSS0044366) as potential key contributors to the zigzag-shaped stem formation. These discoveries lay a robust foundation for future functional genetic mapping and tea plant genetic enhancement.

Citing Articles

Genome-wide identification of F-box-LRR gene family and the functional analysis of CsFBXL13 transcription factor in tea plants.

Dou X, Xie S, Wang J, Shen X, Liu S, Tian N Funct Integr Genomics. 2025; 25(1):57.

PMID: 40063120 DOI: 10.1007/s10142-025-01569-2.

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