Effect of the TetR Family Transcriptional Regulator Sp1418 on the Global Metabolic Network of Saccharopolyspora Pogona

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2020 Feb 13

PMID 32046731

Citations 8

Authors

Haocheng He

Shuangqin Yuan

Jinjuan Hu

Jianming Chen

Jie Rang

Jianli Tang

Zhudong Liu

Ziyuan Xia

Xuezhi Ding

Shengbiao Hu

Liqiu Xia

Affiliations

Soon will be listed here.

Abstract

Background: Saccharopolyspora pogona is a prominent industrial strain due to its production of butenyl-spinosyn, a high-quality insecticide against a broad spectrum of insect pests. TetR family proteins are diverse in a tremendous number of microorganisms and some are been researched to have a key role in metabolic regulation. However, specific functions of TetR family proteins in S. pogona are yet to characterize.

Results: In the present study, the overexpression of the tetR-like gene sp1418 in S. pogona resulted in marked effects on vegetative growth, sporulation, butenyl-spinosyn biosynthesis, and oxidative stress. By using qRT-PCR analysis, mass spectrometry, enzyme activity detection, and sp1418 knockout verification, we showed that most of these effects could be attributed to the overexpression of Sp1418, which modulated enzymes related to the primary metabolism, oxidative stress and secondary metabolism, and thereby resulted in distinct growth characteristics and an unbalanced supply of precursor monomers for butenyl-spinosyn biosynthesis.

Conclusion: This study revealed the function of Sp1418 and enhanced the understanding of the metabolic network in S. pogona, and provided insights into the improvement of secondary metabolite production.

Citing Articles

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Effects of a Pirin-like protein on strain growth and spinosad biosynthesis in Saccharopolyspora spinosa.

Cao L, Zhu Z, Qin H, Xia Z, Xie J, Li X Appl Microbiol Biotechnol. 2023; 107(17):5439-5451.

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The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis in .

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