Bacterioferritin: a Key Iron Storage Modulator That Affects Strain Growth and Butenyl-spinosyn Biosynthesis in Saccharopolyspora Pogona

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2021 Aug 15

PMID 34391414

Citations 8

Authors

Jianli Tang

Zirong Zhu

Haocheng He

Zhudong Liu

Ziyuan Xia

Jianming Chen

Jinjuan Hu

Li Cao

Jie Rang

Ling Shuai

Yang Liu

Yunjun Sun

Xuezhi Ding

Shengbiao Hu

Liqiu Xia

Affiliations

Soon will be listed here.

Abstract

Background: Butenyl-spinosyn, produced by Saccharopolyspora pogona, is a promising biopesticide due to excellent insecticidal activity and broad pesticidal spectrum. Bacterioferritin (Bfr, encoded by bfr) regulates the storage and utilization of iron, which is essential for the growth and metabolism of microorganisms. However, the effect of Bfr on the growth and butenyl-spinosyn biosynthesis in S. pogona has not been explored.

Results: Here, we found that the storage of intracellular iron influenced butenyl-spinosyn biosynthesis and the stress resistance of S. pogona, which was regulated by Bfr. The overexpression of bfr increased the production of butenyl-spinosyn by 3.14-fold and enhanced the tolerance of S. pogona to iron toxicity and oxidative damage, while the knockout of bfr had the opposite effects. Based on the quantitative proteomics analysis and experimental verification, the inner mechanism of these phenomena was explored. Overexpression of bfr enhanced the iron storage capacity of the strain, which activated polyketide synthase genes and enhanced the supply of acyl-CoA precursors to improve butenyl-spinosyn biosynthesis. In addition, it induced the oxidative stress response to improve the stress resistance of S. pogona.

Conclusion: Our work reveals the role of Bfr in increasing the yield of butenyl-spinosyn and enhancing the stress resistance of S. pogona, and provides insights into its enhancement on secondary metabolism, which provides a reference for optimizing the production of secondary metabolites in actinomycetes.

Citing Articles

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Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.

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