Mechanistic Insight for Improving Butenyl-spinosyn Production Through Combined ARTP/UV Mutagenesis and Ribosome Engineering in

Overview

Journal Front Bioeng Biotechnol

Date 2024 Jan 31

PMID 38292303

Authors

Xueli Zhao

Muhammad Hammad Hussain

Ali Mohsin

Zebo Liu

Zhixian Xu

Zhanxia Li

Weiqun Guo

Meijin Guo

Affiliations

Soon will be listed here.

Abstract

Butenyl-spinosyn is a highly effective, wide-spectrum and environmentally-friendly biological insecticide produced by . However, its scale-up is impeded due to its lower titer in wild-type strains. In this work, ARTP/UV mutagenesis and ribosome engineering were employed to enhance the butenyl-spinosyn production, and a stable mutant aG6 with high butenyl-spinosyn yield was successfully obtained. For the first time, the fermentation results in the 5 L bioreactor demonstrated that the butenyl-spinosyn produced by mutant aG6 reached the maximum value of 130 mg/L, almost 4-fold increase over the wild-type strain WT. Furthermore, comparative genomic, transcriptome and target metabolomic analysis revealed that the accumulation of butenyl-spinosyn was promoted by alterations in ribosomal proteins, branched-chain amino acid degradation and oxidative phosphorylation. Conclusively, the proposed model of ribosome engineering combined with ARTP/UV showed the improved biosynthesis regulation of butenyl-spinosyn in .

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