Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene (X) Variants from Bacterial, Fecal, and Environmental Samples

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2020 Feb 12

PMID 32041710

Citations 5

Authors

Yulin Fu

Dejun Liu

Huangwei Song

Zhihai Liu

Haiyang Jiang

Yang Wang

Affiliations

Soon will be listed here.

Abstract

We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of (X) and its variants, including (X1) and (X2) and high-level tigecycline resistance genes (X3), (X4), and (X5). We showed that the real-time PCR assay developed had high linearity ( ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.

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