Dietary Deoxynivalenol Exposure Assessment in University Students from Japan

Overview

Journal Food Saf (Tokyo)

Date 2020 Jan 31

PMID 31998586

Citations 1

Authors

Lei Xia

Yoshiko Sugita-Konishi

Yunyun Gong

Michael Routledge

Affiliations

Soon will be listed here.

Abstract

This study was conducted to give a preliminary estimation of deoxynivalenol (DON) dietary exposure in Japanese university students (n = 30, aged 22-25 years) using a biomarker approach and to examine the correlation between wheat food intake and DON exposure levels. Spot urine samples were collected from 30 students of Azabu University, Tokyo. Urine samples were treated with enzyme digestion (for total DON measurement) and without (for unconjugated DON analysis) before clean-up using an immuno-affinity column and analysis using an LC-MS method, with a C- DON internal standard used for accurate quantification. The limit of detection for this method is 0.5 ng/mL urine. The geometric mean (95% CI) of DON concentration was 2.03 (1.64 - 6.87) ng per mL urine. Ninety of the urine samples had detectable levels of urinary DON. The DON dietary intake exposure estimation suggested that one out of the 30 subjects had an intake of DON that exceeded Joint FAO/WHO Expert Committee on Food Additives (JECFA) provisional maximum tolerable daily intake (PMTDI) level. Mean ratio of free DON to total DON was determined to be 19%. Wheat intake assessed using a basic food frequent questionnaire method did not show a significant correlation with the urinary DON level.

Citing Articles

Subject fields in Food Safety during 10 years.

Yamazoe Y, Yamamoto S, Yoshida M, Kawanishi T, Kumagai S Food Saf (Tokyo). 2021; 9(2):25-31.

PMID: 34249587 PMC: 8254849. DOI: 10.14252/foodsafetyfscj.D-21-00007.

References

Ali N, Blaszkewicz M, Nahid A, Rahman M, Degen G . Deoxynivalenol Exposure Assessment for Pregnant Women in Bangladesh. Toxins (Basel). 2015; 7(10):3845-57. PMC: 4626706. DOI: 10.3390/toxins7103845. View

Turner P, Ji B, Shu X, Zheng W, Chow W, Gao Y . A biomarker survey of urinary deoxynivalenol in China: the Shanghai Women's Health Study. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011; 28(9):1220-3. PMC: 3171645. DOI: 10.1080/19440049.2011.584070. View

Meky F, Turner P, Ashcroft A, Miller J, Qiao Y, ROTH M . Development of a urinary biomarker of human exposure to deoxynivalenol. Food Chem Toxicol. 2002; 41(2):265-73. DOI: 10.1016/s0278-6915(02)00228-4. View

Piekkola S, Turner P, Abdel-Hamid M, Ezzat S, El-Daly M, El-Kafrawy S . Characterisation of aflatoxin and deoxynivalenol exposure among pregnant Egyptian women. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012; 29(6):962-71. DOI: 10.1080/19440049.2012.658442. View

Wells L, Hardie L, Williams C, White K, Liu Y, De Santis B . Deoxynivalenol Biomarkers in the Urine of UK Vegetarians. Toxins (Basel). 2017; 9(7). PMC: 5535143. DOI: 10.3390/toxins9070196. View

Warth B, Sulyok M, Berthiller F, Schuhmacher R, Krska R . New insights into the human metabolism of the Fusarium mycotoxins deoxynivalenol and zearalenone. Toxicol Lett. 2013; 220(1):88-94. DOI: 10.1016/j.toxlet.2013.04.012. View

Solfrizzo M, Gambacorta L, Lattanzio V, Powers S, Visconti A . Simultaneous LC-MS/MS determination of aflatoxin M1, ochratoxin A, deoxynivalenol, de-epoxydeoxynivalenol, α and β-zearalenols and fumonisin B1 in urine as a multi-biomarker method to assess exposure to mycotoxins. Anal Bioanal Chem. 2011; 401(9):2831-41. DOI: 10.1007/s00216-011-5354-z. View

Turner P, Burley V, Rothwell J, White K, Cade J, Wild C . Dietary wheat reduction decreases the level of urinary deoxynivalenol in UK adults. J Expo Sci Environ Epidemiol. 2007; 18(4):392-9. DOI: 10.1038/sj.jes.7500611. View

Srey C, Kimanya M, Routledge M, Shirima C, Gong Y . Deoxynivalenol exposure assessment in young children in Tanzania. Mol Nutr Food Res. 2014; 58(7):1574-80. DOI: 10.1002/mnfr.201400012. View

10.

Ali N, Blaszkewicz M, Degen G . Assessment of deoxynivalenol exposure among Bangladeshi and German adults by a biomarker-based approach. Toxicol Lett. 2016; 258:20-28. DOI: 10.1016/j.toxlet.2016.06.006. View

11.

Wallin S, Gambacorta L, Kotova N, Lemming E, Nalsen C, Solfrizzo M . Biomonitoring of concurrent mycotoxin exposure among adults in Sweden through urinary multi-biomarker analysis. Food Chem Toxicol. 2015; 83:133-9. DOI: 10.1016/j.fct.2015.05.023. View

12.

Katagiri R, Asakura K, Uechi K, Masayasu S, Sasaki S . Iodine Excretion in 24-hour Urine Collection and Its Dietary Determinants in Healthy Japanese Adults. J Epidemiol. 2016; 26(12):613-621. PMC: 5121429. DOI: 10.2188/jea.JE20150245. View

13.

Turner P, Rothwell J, White K, Gong Y, Cade J, Wild C . Urinary deoxynivalenol is correlated with cereal intake in individuals from the United kingdom. Environ Health Perspect. 2008; 116(1):21-5. PMC: 2199283. DOI: 10.1289/ehp.10663. View

14.

Kawamura M, Kimura Y, Takahashi K, Satoh N, Oku K, Adachi T . Relation of urinary sodium excretion to blood pressure, glucose metabolism, and lipid metabolism in residents of an area of Japan with high sodium intake. Hypertens Res. 1998; 20(4):287-93. DOI: 10.1291/hypres.20.287. View

15.

Turner P, Hopton R, Lecluse Y, White K, Fisher J, Lebailly P . Determinants of urinary deoxynivalenol and de-epoxy deoxynivalenol in male farmers from Normandy, France. J Agric Food Chem. 2010; 58(8):5206-12. DOI: 10.1021/jf100892v. View

16.

Berthiller F, Krska R, Domig K, Kneifel W, Juge N, Schuhmacher R . Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. Toxicol Lett. 2011; 206(3):264-7. PMC: 3185207. DOI: 10.1016/j.toxlet.2011.08.006. View

17.

Iwahori T, Ueshima H, Torii S, Saito Y, Kondo K, Tanaka-Mizuno S . Diurnal variation of urinary sodium-to-potassium ratio in free-living Japanese individuals. Hypertens Res. 2017; 40(7):658-664. PMC: 5520391. DOI: 10.1038/hr.2016.187. View

18.

Maul R, Warth B, Kant J, Schebb N, Krska R, Koch M . Investigation of the hepatic glucuronidation pattern of the Fusarium mycotoxin deoxynivalenol in various species. Chem Res Toxicol. 2012; 25(12):2715-7. DOI: 10.1021/tx300348x. View

19.

Knutsen H, Alexander J, Barregard L, Bignami M, Bruschweiler B, Ceccatelli S . Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed. EFSA J. 2020; 15(9):e04718. PMC: 7010102. DOI: 10.2903/j.efsa.2017.4718. View