Silkworm Pupa Oil Attenuates Acetaminophen-induced Acute Liver Injury by Inhibiting Oxidative Stress-mediated NF-κB Signaling

Overview

Journal Food Sci Nutr

Specialty Biotechnology

Date 2020 Jan 30

PMID 31993149

Citations 17

Authors

Xingyao Long

Jiajia Song

Xin Zhao

Yu Zhang

Hongwei Wang

Xinqi Liu

Huayi Suo

Affiliations

Soon will be listed here.

Abstract

Acetaminophen (APAP) overdose causes severe hepatotoxicity and acute liver failure. The current study aims to investigate the protection effects of silkworm pupa oil (SPO) against acute hepatic injury in APAP-exposed Kunming mice. Our results showed that the liver index and the levels of serum alanine transaminase (ALT) and aspartate transaminase (AST) in mice subjected to APAP treatment were decreased by SPO. Supplement of SPO also restored hepatic histopathological alterations induced by APAP. The APAP-induced increase in proinflammatory cytokines, including TNF-α, IL-6, and IL-12, was reversed by SPO, which was mediated by the reduction of nuclear factor (NF)-κB p65 expression and the increase in the expression of IκB-α in liver tissue. Moreover, SPO inhibited APAP-triggered oxidative stress by decreasing MDA level and increasing the activities of SOD and GSH-Px. Collectively, SPO attenuated hepatic injury induced by APAP, which attributed to the suppression of oxidative stress-mediated NF-κB signaling. Our findings suggest that SPO supplementation may be potential strategy against acute hepatic injury.

Citing Articles

Potential uses of silkworm pupae ( L.) in food, feed, and other industries: a systematic review.

Rodriguez-Ortiz L, Hincapie C, Hincapie-Llanos G, Osorio M Front Insect Sci. 2024; 4:1445636.

PMID: 39355451 PMC: 11442273. DOI: 10.3389/finsc.2024.1445636.

The Protective Effect and Mechanism of a Phytochemical Extract from the Wild Vegetable Shutou ( Buch.) against Acetaminophen-Induced Liver Injury in Mice.

Shan M, Ma Q, Sun Y, Gao F, Cai S Foods. 2023; 12(16).

PMID: 37628108 PMC: 10453156. DOI: 10.3390/foods12163109.

Nutritional Value of Silkworm Pupae () with Emphases on Fatty Acids Profile and Their Potential Applications for Humans and Animals.

Habeanu M, Gheorghe A, Mihalcea T Insects. 2023; 14(3).

PMID: 36975939 PMC: 10051144. DOI: 10.3390/insects14030254.

Nutritional aspects and dietary benefits of "Silkworms": Current scenario and future outlook.

Mahanta D, Komal J, Samal I, Bhoi T, Dubey V, Pradhan K Front Nutr. 2023; 10:1121508.

PMID: 36742434 PMC: 9892554. DOI: 10.3389/fnut.2023.1121508.

The Beneficial Effects of Pine Nuts and Its Major Fatty Acid, Pinolenic Acid, on Inflammation and Metabolic Perturbations in Inflammatory Disorders.

Takala R, Ramji D, Choy E Int J Mol Sci. 2023; 24(2).

PMID: 36674687 PMC: 9861571. DOI: 10.3390/ijms24021171.

References

Zhou H, Wang H, Shi K, Li J, Zong Y, Du R . Hepatoprotective Effect of Baicalein Against Acetaminophen-Induced Acute Liver Injury in Mice. Molecules. 2019; 24(1). PMC: 6337302. DOI: 10.3390/molecules24010131. View

Evans S, Rodino K, Adcox H, Carlyon J . Orientia tsutsugamushi uses two Ank effectors to modulate NF-κB p65 nuclear transport and inhibit NF-κB transcriptional activation. PLoS Pathog. 2018; 14(5):e1007023. PMC: 5957444. DOI: 10.1371/journal.ppat.1007023. View

Fu T, Wang S, Liu J, Cai E, Li H, Li P . Protective effects of α-mangostin against acetaminophen-induced acute liver injury in mice. Eur J Pharmacol. 2018; 827:173-180. DOI: 10.1016/j.ejphar.2018.03.002. View

Ghanem C, Perez M, Manautou J, Mottino A . Acetaminophen from liver to brain: New insights into drug pharmacological action and toxicity. Pharmacol Res. 2016; 109:119-31. PMC: 4912877. DOI: 10.1016/j.phrs.2016.02.020. View

Filliol A, Piquet-Pellorce C, Le Seyec J, Farooq M, Genet V, Lucas-Clerc C . RIPK1 protects from TNF-α-mediated liver damage during hepatitis. Cell Death Dis. 2016; 7(11):e2462. PMC: 5260888. DOI: 10.1038/cddis.2016.362. View

Craig D, Bates C, Davidson J, Martin K, Hayes P, Simpson K . Overdose pattern and outcome in paracetamol-induced acute severe hepatotoxicity. Br J Clin Pharmacol. 2011; 71(2):273-82. PMC: 3040549. DOI: 10.1111/j.1365-2125.2010.03819.x. View

Uchida N, Silva-Filho S, Cardia G, Cremer E, Silva-Comar F, Silva E . Hepatoprotective Effect of Citral on Acetaminophen-Induced Liver Toxicity in Mice. Evid Based Complement Alternat Med. 2017; 2017:1796209. PMC: 5499238. DOI: 10.1155/2017/1796209. View

Xie W, Wang M, Chen C, Zhang X, Melzig M . Hepatoprotective effect of isoquercitrin against acetaminophen-induced liver injury. Life Sci. 2016; 152:180-9. DOI: 10.1016/j.lfs.2016.04.002. View

OBrien P, Slaughter M, Swain A, Birmingham J, Greenhill R, Elcock F . Repeated acetaminophen dosing in rats: adaptation of hepatic antioxidant system. Hum Exp Toxicol. 2000; 19(5):277-83. DOI: 10.1191/096032700678815918. View

10.

Long X, Zhao X, Wang W, Zhang Y, Wang H, Liu X . Protective effect of silkworm pupa oil on hydrochloric acid/ethanol-induced gastric ulcers. J Sci Food Agric. 2018; 99(6):2974-2986. DOI: 10.1002/jsfa.9511. View

11.

Khodayar M, Kalantari H, Khorsandi L, Rashno M, Zeidooni L . Betaine protects mice against acetaminophen hepatotoxicity possibly via mitochondrial complex II and glutathione availability. Biomed Pharmacother. 2018; 103:1436-1445. DOI: 10.1016/j.biopha.2018.04.154. View

12.

Zou Y, Hu T, Shi Y, Liao S, Liu J, Mu L . Silkworm pupae oil exerts hypercholesterolemic and antioxidant effects in high-cholesterol diet-fed rats. J Sci Food Agric. 2016; 97(7):2050-2056. DOI: 10.1002/jsfa.8009. View

13.

Devkar S, Kandhare A, Zanwar A, Jagtap S, Katyare S, Bodhankar S . Hepatoprotective effect of withanolide-rich fraction in acetaminophen-intoxicated rat: decisive role of TNF-α, IL-1β, COX-II and iNOS. Pharm Biol. 2016; 54(11):2394-2403. DOI: 10.3109/13880209.2016.1157193. View

14.

Albano E, Rundgren M, Harvison P, Nelson S, Moldeus P . Mechanisms of N-acetyl-p-benzoquinone imine cytotoxicity. Mol Pharmacol. 1985; 28(3):306-11. View

15.

Feng R, Wang Y, Liu C, Yan C, Zhang H, Su H . Acetaminophen-induced liver injury is attenuated in transgenic fat-1 mice endogenously synthesizing long-chain n-3 fatty acids. Biochem Pharmacol. 2018; 154:75-88. DOI: 10.1016/j.bcp.2018.04.019. View

16.

Manosroi A, Boonpisuttinant K, Winitchai S, Manosroi W, Manosroi J . Free radical scavenging and tyrosinase inhibition activity of oils and sericin extracted from Thai native silkworms (Bombyx mori). Pharm Biol. 2010; 48(8):855-60. DOI: 10.3109/13880200903300212. View

17.

Sandilands E, Bateman D . Adverse reactions associated with acetylcysteine. Clin Toxicol (Phila). 2009; 47(2):81-8. DOI: 10.1080/15563650802665587. View

18.

Ishida Y, Kondo T, Tsuneyama K, Lu P, Takayasu T, Mukaida N . The pathogenic roles of tumor necrosis factor receptor p55 in acetaminophen-induced liver injury in mice. J Leukoc Biol. 2003; 75(1):59-67. DOI: 10.1189/jlb.0403152. View

19.

Ding Y, Li Q, Xu Y, Chen Y, Deng Y, Zhi F . Attenuating Oxidative Stress by Paeonol Protected against Acetaminophen-Induced Hepatotoxicity in Mice. PLoS One. 2016; 11(5):e0154375. PMC: 4856301. DOI: 10.1371/journal.pone.0154375. View

20.

Feng D, Wang Y, Xu Y, Luo Q, Lan B, Xu L . Interleukin 10 deficiency exacerbates halothane induced liver injury by increasing interleukin 8 expression and neutrophil infiltration. Biochem Pharmacol. 2008; 77(2):277-84. DOI: 10.1016/j.bcp.2008.09.028. View