Cell Release During Perfusion Reflects Cold Ischemic Injury in Rat Livers

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jan 26

PMID 31980677

Citations 11

Authors

Reinier J de Vries

Casie A Pendexter

Stephanie E J Cronin

Beatriz Marques

Ehab O A Hafiz

Alona Muzikansky

Thomas M van Gulik

James F Markmann

Shannon L Stott

Heidi Yeh

Mehmet Toner

Korkut Uygun

Shannon N Tessier

Affiliations

Soon will be listed here.

Abstract

The global shortage of donor organs has made it crucial to deeply understand and better predict donor liver viability. However, biomarkers that effectively assess viability of marginal grafts for organ transplantation are currently lacking. Here, we showed that hepatocytes, sinusoidal endothelial, stellate, and liver-specific immune cells were released into perfusates from Lewis rat livers as a result of cold ischemia and machine perfusion. Perfusate comparison analysis of fresh livers and cold ischemic livers showed that the released cell profiles were significantly altered by the duration of cold ischemia. Our findings show for the first time that parenchymal cells are released from organs under non-proliferative pathological conditions, correlating with the degree of ischemic injury. Thus, perfusate cell profiles could serve as potential biomarkers of graft viability and indicators of specific injury mechanisms during organ handling and transplantation. Further, parenchymal cell release may have applications in other pathological conditions beyond organ transplantation.

Citing Articles

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