ATG7 is Essential for Secretion of Iron from Ameloblasts and Normal Growth of Murine Incisors During Aging

Overview

Journal Autophagy

Specialty Cell Biology

Date 2019 Dec 28

PMID 31880208

Citations 10

Authors

Supawadee Sukseree

Uwe Yacine Schwarze

Reinhard Gruber

Florian Gruber

Maria Quiles Del Rey

Joseph D Mancias

John D Bartlett

Erwin Tschachler

Leopold Eckhart

Affiliations

Soon will be listed here.

Abstract

The incisors of rodents comprise an iron-rich enamel and grow throughout adult life, making them unique models of iron metabolism and tissue homeostasis during aging. Here, we deleted (autophagy related 7) in murine ameloblasts, i.e. the epithelial cells that produce enamel. The absence of ATG7 blocked the transport of iron from ameloblasts into the maturing enamel, leading to a white instead of yellow surface of maxillary incisors. In aging mice, lack of ATG7 was associated with the growth of ectopic incisors inside severely deformed primordial incisors. These results suggest that 2 characteristic features of rodent incisors, i.e. deposition of iron on the enamel surface and stable growth during aging, depend on autophagic activity in ameloblasts. : ATG5: autophagy related 5; ATG7: autophagy related 7; CMV: cytomegalovirus; Cre: Cre recombinase; CT: computed tomography; FTH1: ferritin heavy polypeptide 1; GFP: green fluorescent protein; KRT5: keratin 5; KRT14: keratin 14; LGALS3: lectin, galactose binding, soluble 3; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; NCOA4: nuclear receptor coactivator 4; NRF2: nuclear factor, erythroid 2 like 2; SQSTM1: sequestosome 1.

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