Combined Action of Piperitenone Epoxide and Antibiotics Against Clinical Isolates of and

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Dec 6

PMID 31803154

Citations 5

Authors

Athanasios Alexopoulos

Athanasios C Kimbaris

Stavros Plessas

Ioanna Mantzourani

Chrysa Voidarou

Olga Pagonopoulou

Christina Tsigalou

Maria Fournomiti

Christos Bontsidis

Elisavet Stavropoulou

Virginia Papaemmanouil

Eugenia Bezirtzoglou

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to determine the antimicrobial efficiency of piperitenone epoxide (PEO) - a principal component of various aromatic plants' essential oil - in combination with various antibiotics against 28 strains of and 10 strains of isolated from clinical samples. essential oil, initially collected by hydrodistillation, was then subjected to flush column chromatography affording PEO of high purity. Minimum inhibitory concentrations of PEO alone and in combination with various concentrations of antibiotics were assessed using the microdilution method. The combined action was estimated calculating the fractional inhibitory concentration (FIC) index from checkerboard assays. Our results showed that the average minimum inhibitory concentration (mg/l) of PEO alone against was 512 ± 364.7 μg/ml, which was significantly higher than 172.8 ± 180.7 μg/ml observed for . From checkerboard assays, FIC values below the 0.5 index, indicating synergy, were observed for 59% of the drugs tested. Twelve percent of FIC index values were between 0.5 and 1, indicating additive effects, while 21% were indifferent. According to our results, PEO could be a promising antimicrobial compound when combined with specific antibiotics and deserves further study.

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