Structural Bases for the Allergenicity of Fra a 1.02 in Strawberry Fruits

Overview

Journal J Agric Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2019 Nov 28

PMID 31774998

Citations 6

Authors

Begona Orozco-Navarrete

Zuzanna Kaczmarska

Florine Dupeux

Maria Garrido-Arandia

Delphine Pott

Araceli Diaz Perales

Ana Casanal

Jose A Marquez

Victoriano Valpuesta

Catharina Merchante

Affiliations

Soon will be listed here.

Abstract

Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient's previous sensitization to the major birch pollen allergen Bet v 1, by which IgE generated in response to Bet v 1 cross-reacts with the structurally related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.

Citing Articles

A systematic review of allergen cross-reactivity: Translating basic concepts into clinical relevance.

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Sensitizations to wheat and strawberry: are they a tangible threat to atopic Egyptian.

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The Structural Flexibility of PR-10 Food Allergens.

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Structure and Zeatin Binding of the Peach Allergen .

Eidelpes R, Hofer F, Rock M, Fuhrer S, Kamenik A, Liedl K J Agric Food Chem. 2021; 69(29):8120-8129.

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Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response.

Orozco-Navarrete B, Song J, Casanal A, Sozzani R, Flors V, Sanchez-Sevilla J Hortic Res. 2021; 8(1):58.

PMID: 33750764 PMC: 7943815. DOI: 10.1038/s41438-021-00492-4.

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