Replication of the Promiscuous Plasmid PLS1: a Region Encompassing the Minus Origin of Replication is Associated with Stable Plasmid Inheritance

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1993 Oct 1

PMID 8232217

Citations 11

Authors

G Del Solar

G Kramer

S Ballester

M Espinosa

Affiliations

Soon will be listed here.

Abstract

Deletion of a region of the promiscuous plasmid pLS1 encompassing the initiation signals for the synthesis of the plasmid lagging strand led to plasmid instability in Streptococcus pneumoniae and Bacillus subtilis. This defect could not be alleviated by increasing the number of copies (measured as double-stranded plasmid DNA) to levels similar to those of the wild-type plasmid pLS1. Our results indicate that in the vicinity of, or associated with the single-stranded origin region of pLS1 there is a plasmid component involved in its stable inheritance. Homology was found between the DNA gyrase binding site within the par region of plasmid pSC101 and the pLS1 specific recombination site RSB.

Citing Articles

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Garay-Novillo J, Ruiz-Maso J, Del Solar G, Barra J Microb Biotechnol. 2024; 17(12):e70060.

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Combining Modules for Versatile and Optimal Labeling of Lactic Acid Bacteria: Two pMV158-Family Promiscuous Replicons, a Pneumococcal System for Constitutive or Inducible Gene Expression, and Two Fluorescent Proteins.

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