Molecular Basis of Senescence Transmitting in the Population of Human Endometrial Stromal Cells

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2019 Nov 6

PMID 31689238

Citations 10

Authors

Anastasiia Griukova

Pavel Deryabin

Alla Shatrova

Elena Burova

Valeria Severino

Annarita Farina

Nikolay Nikolsky

Aleksandra Borodkina

Affiliations

Soon will be listed here.

Abstract

Hormone-regulated proliferation and differentiation of endometrial stromal cells (ESCs) determine overall endometrial plasticity and receptivity to embryos. Previously we revealed that ESCs may undergo premature senescence, accompanied by proliferation loss and various intracellular alterations. Here we focused on whether and how senescence may be transmitted within the ESCs population. We revealed that senescent ESCs may induce paracrine senescence in young counterparts via cell contacts, secreted factors and extracellular vesicles. According to secretome-wide profiling we identified plasminogen activator inhibitor -1 (PAI-1) to be the most prominent protein secreted by senescent ESCs (data are available via ProteomeXchange with identifier PXD015742). By applying CRISPR/Cas9 techniques we disclosed that PAI-1 secreted by senescent ESCs may serve as the master-regulator of paracrine senescence progression within the ESCs population. Unraveled molecular basis of senescence transduction in the ESCs population may be further considered in terms of altered endometrial plasticity and sensitivity to invading embryo, thus contributing to the female infertility curing.

Citing Articles

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