Hindbrain V2a Neurons Pattern Rhythmic Activity of Motor Neurons in a Reticulospinal Coculture

As the capacity to isolate distinct neuronal cell types has advanced over the past several decades, new two- and three-dimensional models of the interactions between different brain regions have expanded our understanding of human neurobiology and the origins of disease. These cultures develop distinctive patterns of activity, but the extent that these patterns are determined by the molecular identity of individual cell types versus the specific pattern of network connectivity is unclear. To address the question of how individual cell types interact , we developed a simplified culture using two excitatory neuronal subtypes known to participate in the reticulospinal circuit: HB9 spinal motor neurons and Chx10 hindbrain V2a neurons. Here, we report the emergence of cell type-specific patterns of activity in culture; on their own, Chx10 neurons developed regular, synchronized bursts of activity that recruited neurons across the entire culture, whereas HB9 neuron activity consisted of an irregular pattern. When these two subtypes were cocultured, HB9 neurons developed synchronized network bursts that were precisely correlated with Chx10 neuron activity, thereby recreating an aspect of Chx10 neurons' role in driving motor activity. These bursts were dependent on AMPA receptors. Our results demonstrate that the molecular classification of the neurons comprising networks is a crucial determinant of their activity. It is therefore possible to improve both the reproducibility and the applicability of neurobiological and disease models by carefully controlling the constituent mixtures of neuronal subtypes.