Transcriptomic Profile of Cystic Fibrosis Airway Epithelial Cells Undergoing Repair

Overview

Journal Sci Data

Specialty Science

Date 2019 Oct 31

PMID 31664037

Citations 12

Authors

Alice Zoso

Aderonke Sofoluwe

Marc Bacchetta

Marc Chanson

Affiliations

Soon will be listed here.

Abstract

Pathological remodeling of the airway epithelium is commonly observed in Cystic Fibrosis (CF). The different cell types that constitute the airway epithelium are regenerated upon injury to restore integrity and maintenance of the epithelium barrier function. The molecular signature of tissue repair in CF airway epithelial cells has, however, not well been investigated in primary cultures. We therefore collected RNA-seq data from well-differentiated primary cultures of bronchial human airway epithelial cells (HAECs) of CF (F508del/F508del) and non-CF (NCF) origins before and after mechanical wounding, exposed or not to flagellin. We identified the expression changes with time of repair of genes, the products of which are markers of the different cell types that constitute the airway epithelium (basal, suprabasal, intermediate, secretory, goblet and ciliated cells as well as ionocytes). Researchers in the CF field may benefit from this transcriptomic profile, which covers the initial steps of wound repair and revealed differences in this process between CF and NCF cultures.

Citing Articles

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References

Schiller K, Maniak P, OGrady S . Cystic fibrosis transmembrane conductance regulator is involved in airway epithelial wound repair. Am J Physiol Cell Physiol. 2010; 299(5):C912-21. PMC: 2980302. DOI: 10.1152/ajpcell.00215.2010. View

Voynow J, Fischer B, Roberts B, Proia A . Basal-like cells constitute the proliferating cell population in cystic fibrosis airways. Am J Respir Crit Care Med. 2005; 172(8):1013-8. DOI: 10.1164/rccm.200410-1398OC. View

Itokazu Y, Pagano R, Schroeder A, OGrady S, Limper A, Marks D . Reduced GM1 ganglioside in CFTR-deficient human airway cells results in decreased β1-integrin signaling and delayed wound repair. Am J Physiol Cell Physiol. 2014; 306(9):C819-30. PMC: 4010808. DOI: 10.1152/ajpcell.00168.2013. View

Plasschaert L, Zilionis R, Choo-Wing R, Savova V, Knehr J, Roma G . A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Nature. 2018; 560(7718):377-381. PMC: 6108322. DOI: 10.1038/s41586-018-0394-6. View

Treutlein B, Brownfield D, Wu A, Neff N, Mantalas G, Espinoza F . Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature. 2014; 509(7500):371-5. PMC: 4145853. DOI: 10.1038/nature13173. View

Crespin S, Bacchetta M, Bou Saab J, Tantilipikorn P, Bellec J, Dudez T . Cx26 regulates proliferation of repairing basal airway epithelial cells. Int J Biochem Cell Biol. 2014; 52:152-60. DOI: 10.1016/j.biocel.2014.02.010. View

Zoso A, Sofoluwe A, Bacchetta M, Chanson M . Transcriptomic profile of cystic fibrosis airway epithelial cells undergoing repair. Sci Data. 2019; 6(1):240. PMC: 6820749. DOI: 10.1038/s41597-019-0256-6. View

Hajj R, Lesimple P, Nawrocki-Raby B, Birembaut P, Puchelle E, Coraux C . Human airway surface epithelial regeneration is delayed and abnormal in cystic fibrosis. J Pathol. 2006; 211(3):340-50. DOI: 10.1002/path.2118. View

Adam D, Bilodeau C, Sognigbe L, Maille E, Ruffin M, Brochiero E . CFTR rescue with VX-809 and VX-770 favors the repair of primary airway epithelial cell cultures from patients with class II mutations in the presence of Pseudomonas aeruginosa exoproducts. J Cyst Fibros. 2018; 17(6):705-714. DOI: 10.1016/j.jcf.2018.03.010. View

10.

Crespin S, Bacchetta M, Huang S, Dudez T, Wiszniewski L, Chanson M . Approaches to study differentiation and repair of human airway epithelial cells. Methods Mol Biol. 2011; 742:173-85. DOI: 10.1007/978-1-61779-120-8_10. View

11.

Revinski D, Zaragosi L, Boutin C, Ruiz-Garcia S, Deprez M, Thome V . CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. Nat Commun. 2018; 9(1):4668. PMC: 6220262. DOI: 10.1038/s41467-018-06768-z. View

12.

Trinh N, Bardou O, Prive A, Maille E, Adam D, Lingee S . Improvement of defective cystic fibrosis airway epithelial wound repair after CFTR rescue. Eur Respir J. 2012; 40(6):1390-400. DOI: 10.1183/09031936.00221711. View

13.

Wansleeben C, Barkauskas C, Rock J, Hogan B . Stem cells of the adult lung: their development and role in homeostasis, regeneration, and disease. Wiley Interdiscip Rev Dev Biol. 2013; 2(1):131-48. DOI: 10.1002/wdev.58. View

14.

Cohen T, Prince A . Cystic fibrosis: a mucosal immunodeficiency syndrome. Nat Med. 2012; 18(4):509-19. PMC: 3577071. DOI: 10.1038/nm.2715. View

15.

Whitsett J, Alenghat T . Respiratory epithelial cells orchestrate pulmonary innate immunity. Nat Immunol. 2014; 16(1):27-35. PMC: 4318521. DOI: 10.1038/ni.3045. View

16.

Puchelle E, Zahm J, Tournier J, Coraux C . Airway epithelial repair, regeneration, and remodeling after injury in chronic obstructive pulmonary disease. Proc Am Thorac Soc. 2006; 3(8):726-33. DOI: 10.1513/pats.200605-126SF. View