CDC20B is Required for Deuterosome-mediated Centriole Production in Multiciliated Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Nov 9

PMID 30405130

Citations 33

Authors

Diego R Revinski

Laure-Emmanuelle Zaragosi

Camille Boutin

Sandra Ruiz-Garcia

Marie Deprez

Virginie Thome

Olivier Rosnet

Anne-Sophie Gay

Olivier Mercey

Agnes Paquet

Nicolas Pons

Gilles Ponzio

Brice Marcet

Laurent Kodjabachian

Pascal Barbry

Affiliations

Soon will be listed here.

Abstract

Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, which hosts microRNA-449abc. We show that CDC20B protein associates to deuterosomes and is required for centriole release and subsequent cilia production in mouse and Xenopus MCCs. CDC20B interacts with PLK1, a kinase known to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescues centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules.

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